Streptomyces albovinaceus DSM 40136中环二肽的盐胁迫发掘

The Mining of the Cyclodipeptide from Streptomyces albovinaceus DSM 40136 Through Salt Stress

该文选择陆生放线菌Streptomyces albovinaceus DSM 40136,结合菌株肽类次级代谢产物生物合成基因簇的分析,利用盐胁迫策略对其进行产物发掘,成功地利用添加海盐质量分数为3%的M-ISP4培养基激活菌株生产出具有多种生物学活性的环二肽Cyclo(L-Pro-L-Leu).该产物的发掘表明:利用海盐胁迫策略可有效应用于陆生放线菌次级代谢潜能的激活,且新产物的发掘也为环二肽生物合成研究及代谢工程改造提供了良好的出发菌株.

In this paper,the Streptomyces albovinaceus DSM 40136 with detail genomic sequence is selected to cultivated by salt stress.After the comparison analysis of the fermentation extract,the strain can be activated to produce Cyclo(L-Pro-L-Leu)in the cultivated by M-ISP4 medium added 3% sea salt.This activation of natural product biosynthesis indicates that the feasible of salt stress applies in secondary metabolites development derived from terrestrial actinomycetes.Additionally,the S.albovinaceus DSM 40136,new Cyclo(L-Pro-L-Leu)producer,are also new strain deserved in future biosynthesis mechanism research and metabolic engineering modification.