An Efficient in Vitro Plant Regeneration System from Cotyledon of Kenaf(Hibiscus cannabinus L.)

红麻子叶外植体高效再生体系的建立

[Objective]This study aims to develop an efficient regeneration system for kenaf(Hibiscus cannabinus L.),which is expected to lay a basis for breeding via genetic modification and improving the fibre yield.[Method]The influence of kenaf variety,hormone for callus induction,and explant type on the regeneration was examined.[Result]The optimal variety and explant for the regeneration were K89 and cotyledon,respectively.The 6-benzylaminopurine(6-BA)was suitable for callus induction and the optimum concentration was 2 mg/L.In addition,with this cytokinin,1.7~4.0 adventitious buds were produced,and 27.7%~38.3% of the adventitious buds could grow to plants.Adventitious roots can be induced with 0.2 mg/L naphthylacetic acid(NAA)and 0.1 mg/L indole-3-acetic acid(IAA).[Conclusion]Cotyledon of K89 had huge potential for the regeneration of kenaf.

为了建立红麻高效再生体系,更好地开展红麻基因工程育种和基因编辑育种,提高红麻纤维产量,本研究对红麻品种、诱导愈伤组织产生的激素种类、外植体类型进行了筛选,鉴定。研究结果表明红麻再生能力最好的品种为K89,再生能力最好的外植体是子叶。细胞分裂素6-苄氨基嘌呤(6-BA)可以诱导愈伤组织产生,最适浓度为2 mg/L,诱导愈伤组织产生1.7~4.0个不定芽,不定芽获得再生植株的效率为27.7%~38.3%;0.2mg/L萘乙酸(NAA)和0.1 mg/L生长素(IAA)可以诱导再生植株不定根的产生。以上结果表明以红麻品种K89的子叶为外植体能够有效诱导红麻植株的再生。