Biological Characteristics and Genome of Pardosa pseudoannulata

拟环纹豹蛛生物学特性及其基因组调查分析

At the moment,little is found on the whole genomes of spiders in public databases.To this end,this study aimed to analyze the morphology,biological characteristics,and genome of Pardosa pseudoannulata,which was expected to add genome information of spiders.To be specific,Illumina sequencing was used to sequence the genome of this spider species,and K-mer(K=17)analysis to estimate the size,heterozygosity,and repetitiveness of the genome.More than 142.11 Gb high-quality reads were obtained,which were assembled into 3508.13 Mb contigs(N50=1035 bp)and 3542.56 Mb scaffolds(N50=1258 bp).Two peaks were found in the 17-mer distribution map.The genome of P.pseudoannulata was 4696.86 Mb,with the heterozygosity of 0.21%and 84.32%of repetitive sequences.This indicated that repetitive sequences were highly abundant in the genome of P.pseudoannulata and multi-generation selfing before genome sequencing can reduce the repetitiveness.In addition,NOVOheter can be used for genome assembly.This study layed a basis for the publication of spider genome data and provided a reference for the research on spider genomics.

目前蜘蛛的全基因组尚未在公共数据库中发表,仍处于不断探索阶段。为丰富蜘蛛基因组的研究内容,进一步促进蜘蛛基因组序列在公共数据库中的发表和使用,本研究对拟环纹豹蛛(Pardosa pseudoannulata)的形态、生物特性和基因组进行了分析。利用Illumina高通量测序技术对其进行基因组测序,采用K-mer 17分析法预测其基因组大小、杂合度和重复序列等基因组特征。研究表明,从基因组中获得了超过142.11 Gb的高质量数据,这些数据被组装成3508.13 Mb的contigs和3542.56 Mb的scaffolds。contigs的N50长度和总长度分别为1035 bp和3508.13 Mb,scaffolds的N50长度和总长度分别为1258bp和3542.56 Mb。拟环纹豹蛛的基因组大小为4696.86 Mb,呈现出两个明显的峰值,其杂合子率为0.21%,重复率为84.32%。拟环纹豹蛛的基因组测序结果具有高度重复性,这表明在基因组测序之前的多代自交有利于降低重复性,并且NOVOheter可用于基因组组装。本研究为促进蜘蛛基因组数据的发表提供了基础,同时也为蜘蛛的基因组学研究提供了参考价值。