甲连盆腔胶囊对慢性盆腔炎性疾病大鼠血液流变学与病理形态学的影响

Effects of Jialian Penqiang Capsule on hemorheological and pathomorphological indices in rats with chronic pelvic inflammatory disease

目的观察甲连盆腔胶囊对慢性盆腔炎性疾病(CPID)模型大鼠血液流变学和病理形态学的影响,探讨甲连盆腔胶囊治疗CPID的作用及其机制。方法将24只大鼠随机分为两组(空白组12只,假手术组12只),余大鼠使用苯酚胶浆诱导CPID模型,使用随机数字表法将造模成功的59只大鼠分为模型组,甲连盆腔胶囊低、中、高剂量组和妇科千金胶囊组,每日分别予以灌胃生理盐水,甲连盆腔胶囊低、中、高剂量(0.375 g/kg、0.75 g/kg、1.5 g/kg)和妇科千金胶囊(0.25 g/kg),连续灌胃21 d。分别在灌胃第14天和第21天时检测血液流变学指标,并对治疗21 d的大鼠子宫组织行HE染色观察其病理形态学变化。结果与空白组和假手术组比较,模型组大鼠血液流变学各指标均上升(P<0.05);而甲连盆腔胶囊高剂量组在治疗第14天和第21天的血液流变学指标均降低(P<0.05)。结论甲连盆腔胶囊可通过调节血液流变学从而起到治疗CPID的作用。

Objective To observe the effects of Jialian Penqiang Capsule on the hemorheological and pathomorphological indices of rats with chronic pelvic inflammatory disease(CPID),and explore the effects and mechanisms of Jialian Penqiang Capsule in the treatment of CPID.Methods Twenty-four rats were randomly divided into two groups(12 in the blank group and 12 in the sham operation group).The rest rats were induced by phenol glue into the CPID model.59 rats were successfully modeled and they were were divided into the model group,the low-dose,middle-dose and high-dose Jialian Penqiang Capsule groups,and the Fuke Qianjin Capsule group according to the random number table.The rat model in corresponding groups was given normal saline,with low-dose(0.375 g/kg),middle-dose(0.75 g/kg),high-dose(1.5 g/kg)of Jialian Penqiang Capsule and Fuke Qianjin Capsule(0.25 g/kg),respectively,once a day for 21 days respectively.Blood samples were collected on the 14th and 21st day after intragastric administration to detect the hemorheological indices,and HE staining was performed on the uterus tissue of rats treated for 21 days to observe the pathological changes.Results Compared with the blank group and the sham operation group,the hemorheological indices increased in the model group(P<0.05),while the hemorheological indices decreased in the high-dose Jialian Penqiang Capsule group on the 14th and 21st day of treatment(P<0.05).Conclusion Jialian Penqiang Capsule can treat CPID by regulating hemorheology.