基于细菌磁小体-核酸适配体的赭曲霉毒素A富集纯化方法研究

An Enrichment and Purification Method of Ochratoxin a Basing on Aptamer Modified Bacterial Magnetosomes

细菌磁小体是一类由四氧化三铁或四硫化三铁及其外包被的生物膜组成的,粒径在30~120 nm之间的纳米磁性颗粒,其外包被有富含羟基、亚氨基等活性基团的囊泡,为生物/化学修饰提供了基础。本研究基于细菌磁小体的磁响应及易修饰特性,利用戊二醛作为双功能试剂,构建了细菌磁小体-戊二醛-核酸适配体复合物,用于富集、纯化样品中赭曲霉毒素A。结果表明,富集纯化方法对OTA的最高吸附率为96%,回收率为96%。方法不仅操作简单、快速、高效,而且有机溶剂用量少,为细菌磁小体的应用及OTA的富集纯化提供了新的研究思路。

Bacterial magnetosomes,consisting of magnetite(Fe_(3)O_(4))or greigite(Fe_(3)S_(4))and enveloped with biomembrane,are a type magnetic nano-particle with a diameter of 30~120 nm.The exoperidium of the bacterial magnetosomes has the vesicle of the active groups with abundant oxhydryl and imino,providing a basis for bio-modification/chemical modification.In the present study,the magnetic response and the feature of being easily modified were researched.Glutaric dialdehyde was used as bi-functional reagent to combine the magnetosomes and nucleic acid aptamer for enrichment and purification of ochratoxin A in the samples.The results suggested that the adsorption rate of this method for OTA was 99%,and the recovery rate was 96%.In conclusion,this method presented a simple,efficient,as well as organic solvent reduction methods for OTA enrichment and purification,which provided a new idea in bacterial magnetosomes application and OTA purification.