高效液相色谱法测定牛羊组织中碘醚柳胺残留量

Determination of Rafoxanide Residues in the Tissue of Cattle and Sheep by Liquid Chromatography Method

建立高效液相色谱荧光检测法测定牛羊组织中碘醚柳胺残留量。牛羊组织中残留的碘醚柳胺,经乙腈-丙酮溶液提取,阴离子交换固相萃取柱净化,高效液相色谱-荧光法测定,外标法定量。碘醚柳胺在10~5000 ng/mL的浓度范围内呈现良好的线性关系,碘醚柳胺在牛羊肌肉、肝脏、肾脏、脂肪中的检测限均为2.5μg/kg^(-1),定量限均为5μg/kg^(-1),牛羊肌肉、肝脏、肾脏、脂肪中15~500 ng/g添加浓度范围内的回收率为77.4%~93.8%,批内批间RSD值均<15%。

A method of high performance liquid chromatography for determination of rafoxanide in the tissue of cattle and sheep was developed. The samples were extracted with acetonitrile-acetone(80+20), and cleaned up with MAX solid phase extraction cartridg.The separation of the target was carried out on a Ccolumn by elution with acetonitrile-0.1% formic acid(85∶15) as mobile phase, The results showed that correlation coefficient was 0.9999.The LOD and LOQ were 2.5 μg·kg^(-1)and 5.0 μg·kg^(-1).The average recoveries were between 77.4% and 93.8% at spiked levels of 15~500 μg·kg^(-1).The RSD were below 15%.The real sample tests showed this method can be used for the sensitive and accurate determination of rafoxanide residues in the tissue of cattle and sheep.