载锌基沸石咪唑的疏基化海藻酸钙复合水凝胶治疗大鼠牙周炎的实验研究

Application of sulfylated calcium alginate composite hydrogel loaded with zinc-based zeolite imidazole for the treatment of periodontitis

目的:探讨载有锌基沸石咪唑的黏附性疏基化海藻酸钙复合水凝胶(ZIF-8@CHA-SH)治疗牙周炎的可行性。方法:进行ZIF-8@CHA-SH的制备与表征,观察形貌结构和分析化学组成。采用平板涂布法和扫描电镜检测抗菌性能,利用CCK-8和活/死细胞荧光染色法评估ZIF-8@CHA-SH对牙龈成纤维细胞的毒性,通过动物实验验证ZIF-8@CHA-SH的抗炎作用。采用GraphPad Prism 7.0软件对数据进行统计学分析。结果:通过扫描电镜、能谱分析、傅里叶红外光谱、X射线衍射证明,ZIF-8@CHA-SH成功合成。ZIF-8@CHA-SH具有优异的抗菌能力,对大肠杆菌、金黄色葡萄球菌和变异链球菌的抗菌率分别为(99.88±0.12)%、(99.81±0.32)%和(95.53±3.08)%(P<0.001);ZIF-8@CHA-SH具有良好的生物安全性,与人牙龈成纤维细胞共培养5天后的细胞存活率为(91.64±3.66)%(P=0.6)。体内实验表明,ZIF-8@CHA-SH可减少大鼠牙周炎的炎细胞浸润和促炎因子IL-6的表达,具有一定的抗炎能力。结论:ZIF-8@CHA-SH生物性能优异,可有效抑制细菌引起的牙周炎,为未来牙周疾病的治疗提供了新的途径。

PURPOSE:To explore the feasibility of adhesive thiomer calcium alginate composite hydrogel(ZIF-8@CHASH)containing metal-organic framework for the treatment of periodontitis in rats.METHODS:Preparation and characterization of ZIF-8@CHA-SH were performed,and the morphology and chemical composition were observed.The antibacterial properties were detected by plate coating method and scanning electron microscope.CCK-8 and live/dead cell fluorescence staining were used to evaluate the toxicity of ZIF-8@CHA-SH on gingival fibroblasts.The anti-inflammatory and tissue repair promoting effects of ZIF-8@CHA-SH were verified by animal experiments.GraphPad Prism 7.0 software was used for statistical analysis.RESULTS:The successful synthesis of ZIF-8@CHA-SH was proved by scanning electron microscopy,energy spectrum analysis,Fourier transform infrared spectroscopy and X-ray diffraction.ZIF-8@CHA-SH had excellent antibacterial ability,and the antibacterial rates against Escherichia coli,Staphylococcus aureus and Streptococcus mutans were(99.88±0.12)%,(99.81±0.32)%and(95.53±3.08)%,respectively(P<0.001).The cell viability rate of ZIF-8@CHA-SH was(91.64±3.66)%after 5 days of co-culture with human gingival fibroblasts(P=0.6).In vivo experiments showed that ZIF-8@CHA-SH could reduce the infiltration of inflammatory cells and the expression of pro-inflammatory factor IL-6 in rats with periodontitis.CONCLUSIONS:ZIF-8@CHA-SH has excellent biological performance,which can effectively treat periodontitis caused by bacteria,and provids a new strategy for the treatment of periodontal diseases in the future.