玫瑰花中新唢呐草素Ⅰ的分离、含量测定和体外活性研究

Separation,content determination and in vitro activities of tellimagrandinⅠfrom Rosa rugosa

采用葡聚糖凝胶柱层析和半制备高效液相色谱法,从阿尔山玫瑰花提取物中分离纯化得到纯度较高(95.48%)的新唢呐草素I,并鉴定其化学结构;同时采用高效液相色谱法测定了11种不同产地的玫瑰花中新唢呐草素I含量,其含量为0.7%~7.4%;体外活性实验结果表明,新唢呐草素I对α-葡萄糖苷酶有较强的抑制活性,IC为10.0μg/mL,并不同产地玫瑰花提取物均显示抑制活性;新唢呐草素I也具有较强的DPPH自由基清除能力,半数清除率IC为8.1μg/mL,不同产地玫瑰花提取物均有清除DPPH自由基能力。而随着玫瑰花中新唢呐草素I含量的增加,其α-葡萄糖苷酶抑制活性和清除DPPH自由基能力有增强的趋势,但无显著性差异;本研究为玫瑰花提取物的降低餐后血糖活性成分的研究提供了实验依据。

A compound tellimagrandin I with high purity(95.48%)was isolated and prepared from Aershan Rosa rugosa extract using Sephadex LH-20 column chromatography and semi-preparative high performance liquid chromatography,and its chemical structure was identified;the content of the tellimagrandin I in R.rugosa which was determined by HPLC and its contents in 11 kinds of R.rugosa was from 0.7%to 7.4%.In the in vitro activities test,tellimagrandin I showed stronger inhibitory effect onα-glucosidase and the ICvalues was 10.0μg/mL.The extracts of R.rugosa from different areas also showed better inhibitory activities onα-glucosidase.Also,tellimagrandin I showed stronger DPPH free radical scavenging activity,and the ICvalues was 8.1μg/mL.The extracts of R.rugosa from different areas all showed DPPH free radical scavenging activities.With the contents of the tellimagrandin I in extracts of R.rugosa from different areas,the correspondingα-glucosidase inhibitory and DPPH free radical scavenging activities were tending to increase,but there was no significant correlation.This study provided a reference for clarifying the active ingredients of R.rugosa extract for lowering postprandial blood sugar,and provided a basis for the development and utilization of R.rugosa.