九香虫内生曲霉菌次生代谢产物及其抗菌活性研究

Study on the secondary metabolites of endophytic fungus Aspergillus sp.from Aspongopus chinensis and their antimicrobial activity

本文对一株来自九香虫肠道的内生曲霉菌(Aspergillus sp.)的代谢产物进行研究。利用硅胶柱色谱、MCI柱色谱、凝胶柱色谱及半制备液相等方法对该菌的大米固体发酵浸膏进行分离纯化,从中分离得到6个化合物。通过核磁共振和质谱技术对这6个化合物结构进行解析,包括1个新化合物:flavipone(1)和5个已知化合物:citreovirenone(2)、kojic acid(3)、methyl chloroasterrate(4)、aspulvinone P(5)、aspulvinone Q(6),其中化合物1和2首次从曲霉菌中分离得到。活性测试显示化合物3和4具有一定的抑菌作用,MIC值在35.5到67.5μg/mL之间。

The metabolites of an endophytic Aspergillus sp.from the intestine of Aspongopus chinensis were studied in tihis paper.Six compounds were isolated from rice solid fermentation extract by silica gel column chromatography,MCI column chromatography,gel column chromatography and semi preparative liquid method.The structures of the six compounds were analyzed by NMR and mass spectrometry,including a new compound flavion(1)and five known compounds citreovirenone(2),kojic acid(3),methyl chloroacetate(4),aspulvinone P(5)and aspulvinone Q(6).Compounds 1 and 2 were isolated from Aspergillus for the first time.Compounds 3 and 4 exhibited weak antimicrobial effects with MIC values ranging from 35.5 to 67.5μg/mL.