天山假狼毒的化学成分研究

Chemical constituents from the Stelleropsis tianschanica

研究天山假狼毒Stelleropsis tianschanica的化学成分。运用柱色谱的方法对其进行分离纯化,结合波谱技术与理化性质鉴定化合物结构。本文共从天山假狼毒95%甲醇提取物中分离得到了15个化合物,其中二萜类3个、苯丙素类4个、酚酸类4个、其他类化合物4个,分别鉴定为excoecariatoxin(1)、12-羟基瑞香毒素(2)、2,5-二甲氧基对苯醌(3)、rutamontine(4)、scorpinone(5)、dihydrosyringenin(6)、N-苯乙基乙酰胺(7)、松脂醇(8)、马台树脂醇(9)、vesiculosin(10)、threo-8S-7-methoxysyringylglycerol(11)、cyclo(D)-pro-(D)-leu(12)、threo-3-(4-hydroxy-3,5-dimethoxyphenyl)-3-ethoxypropane-1,2-diol(13)、(-)-丁香素脂酚(14)、threo-1-(4-hydroxy-3-methoxyphenyl)-2-{4-[(E)-3-hydroxy-1-propenyl]-2-methoxyphenoxy}-1,3-propanediol(15),其中化合物1~7、12~15均为首次从假狼毒属植物中分离得到。化合物1~15进行细胞毒活性筛选,结果显示化合物1、2、3和10在50μmol/L时对HGC-27细胞株具有较强的抑制活性,抑制率依次为92.0%、86.8%、87.28%、76.34%。其对HGC-27细胞的IC值分别为14.06、15.23、17.28、27.1μmol/L。

The chemical constituents of Stelleropsis tianschanica were separated and purified by column chromatography,and the structures were identified by spectra analysis combined with physical and chemical properties.Finally,a total of 15 compounds were isolated from 95%methanol extract of S.tianschanica,including three diterpenoids compounds,four phenylpropanoids compounds,four phenolic acids compounds,and the other four compounds.The structures were identified as excoecariatoxin(1),12-hydroxydaphnetoxin(2),2,5-dimethoxybenzoquinone(3),rutamontine(4),scorpinone(5),dihydrosyringenin(6),N-(2-phenylethyl)acetamide(7),pinoresinol(8),matairesinol(9),vesiculosin(10),threo-8S-7-methoxysyringylglycerol(11),cyclo(D)-pro-(D)-leu(12),threo-3-(4-hydroxy-3,5-dimethoxyphenyl)-3-ethoxypropane-1,2-diol(13),(-)-syringaresinol(14),threo-1-(4-hydroxy-3-methoxyphenyl)-2-{4-[(E)-3-hydroxy-1-propenyl]-2-methoxyphenoxy}-1,3-propanediol(15).Compounds 1-7,12-15 are isolated from the Stelleropsis genus for the first time.In addition,cytotoxic effects of compounds 1-15 were screened on HGC-27 gastric cancer cell lines.The results showed that compounds 1,2,3 and 10 were in the range of 50μmol/L had strong inhibitory activity,and the inhibition rates were 92.0%,86.8%,87.28%and 76.34%,respectively,and their ICvalues were 14.06,15.23,17.28,27.1μmol/L.