镉胁迫下姬松茸菌株转录组测序及分析

Sequencing and Analysis of Agaricus blazei Mycelium Transcriptome Under Cadmium Stress

为探明姬松茸菌株对重金属镉的富集机理,以前期筛选出的镉高、低富集姬松茸菌株(J11、J4)为材料进行2 mg/L的镉胁迫处理,以未添加外源镉作对照,采用Illumina高通量测序技术对其菌丝样品进行转录组测序分析。结果表明:差异表达基因主要富集在类固醇生物合成、抗生素的生物合成、碳代谢、谷胱甘肽代谢、内质网的蛋白质加工、甲烷代谢、淀粉和蔗糖的代谢等通路;其中,类固醇生物合成、抗生素的生物合成途径上调;甲烷代谢、淀粉和蔗糖代谢、碳代谢、谷胱甘肽、乙醛酸和二羧酸等代谢途径下调。分析认为碳代谢、谷胱甘肽代谢可能在姬松茸菌株响应镉胁迫方面起重要的作用。

In order to explore the mechanism of cadmium enrichment in Agaricus blazei Murill,the Illumina high-throughput sequencing technology was used for the transcriptome sequencing of Agaricus blazei mycelium samples by using two preselected strains J11 and J4(low and high cadmium-enriched).The control group without exogenous cadmium addition and the treatment group under 2 mg/L cadmium stress were set in our experiment.The results showed that the differentially-expressed genes were mainly concentrated in steroid and antibiotic biosynthesis,carbon and glutathione metabolism,endoplasmic reticulum protein processing,methane and starch and sucrose metabolism,and other pathways.Among them,biosynthetic pathways of steroid and antibiotics were up-regulated,while metabolism pathways of methane,starch and sucrose,carbon,glutathione,glyoxylic acid and dicarboxylic substances were down-regulated.These results support that metabolism of carbon and glutathione may play an important role in the response of Agaricus blazei mycelium to cadmium stress.