响应面法优化刺葡萄果实花旗松素提取工艺

Optimization of Extraction Technology of Taxifolin from Fruit of Vitis davidii by Response Surface Methodology

目的:为充分利用刺葡萄资源,明确刺葡萄果实不同组织的花旗松素分布规律。方法:采用超声波辅助提取花旗松素,利用HPLC-MS/MS技术进行花旗松素检测,并通过单因素实验及响应面优化了刺葡萄果皮花旗松素提取工艺。结果:刺葡萄果皮中花旗松素含量为3.63±0.18 mg/kg,显著(P<0.05)高于种子的1.74±0.13 mg/kg和果肉的0.35±0.02 mg/kg。单因素实验表明,刺葡萄果皮中花旗松素提取量在乙醇浓度为50%、提取时间在4 h、提取温度50℃时达到最高值。响应面优化刺葡萄果皮花旗松素的最佳提取工艺为乙醇含量49%、浸泡温度30℃、浸泡时间1 h、超声功率100 W、超声时间0.5 h、提取时间4 h、提取温度51℃,花旗松素提取量为3.587 mg/kg。结论:中国野生刺葡萄中含有比较丰富的花旗松素,本研究结果为刺葡萄果皮的加工与利用提供理论依据。

Objective: To clarify the distribution patterns and make full use of Chinese wild resources Vitis davidii fruit in different tissues. Methods: Ultrasonic-assisted extraction and HPLC-MS/MS techniques were used for the determination of taxifolin. The response surface analysis was used to optimize the extraction process of taxifolin and the single factor test.Results: The taxifolin content in fruit peel was 3.63±0.18mg/kg, significantly(P<0.05) higher than that in seed(1.74±0.13mg/kg) and pulp(0.35±0.02mg/kg). The extraction efficiency was the highest when the ethanol concentration was 50%, and the extraction time was proportional to the extraction amount within 4 h. The dynamic equilibrium reached between extraction time and extraction amount after 4 h. The extraction amount reached the peak at 50 ℃. The optimal extraction conditions were finally determined with ethanol 49%, soaking temperature 30 ℃, soaking time 1 h, ultrasonic power 100 W, ultrasonic time 0.5 h, extraction time 4 h and extraction temperature 51 ℃. With the optimal conditions, the taxifolin extraction amount was up to 3.587 mg/kg. Conclusion: Chinese wild Vitis davidii fruit was rich in taxifolin, this study would provide a theoretical basis for the processing and utilization of Vitis davidii.