金霉素分子印迹电化学传感器的制备与应用

Preparation and application of chlortetracycline electrochemical sensor based on molecularly imprinting technique

金霉素(CTC)的滥用给自然环境和人类健康带来了严重的不良影响。建立了一种简便、经济、高效的CTC分子印迹电化学传感器。该传感器的分子印迹膜由邻苯二胺在还原型氧化石墨烯-聚乙烯亚胺复合物(RGO-PEI)修饰的玻碳电极上电聚合而成。采用扫描电子显微镜、红外吸收光谱和紫外可见吸收光谱对RGO PEI复合材料进行了表征。RGO-PEI复合材料的高比表面积和丰富的氨基基团提高了该传感器检测的灵敏度和稳定性。在优化条件下,该传感器对CTC浓度响应的线性范围为(5.0×10^(-7))~(1.0×10^(-4))mol/L,检测限为1.67×10^(-7)mol/L(信噪比,S/N=3)。此外,该传感器对卡那霉素、土霉素和盐酸多西环素等干扰物质的响应很小,可用于实际样品中CTC的检测,回收率为102.7%~104.7%,是一种简单、高效的电化学方法。

The abuse of chlortetracycline(CTC)has brought serious adverse effects on the natural environment and human health.In this paper,a simple,economical and efficient molecularly imprinted electrochemical sensor for CTC was developed.The sensor was constructed by the electropolymerization of o-phenylenediamine on a reduced graphene oxide-polyethyleneimine complex(RGO-PEI)modified glassy carbon electrode.The RGO-PEI composite was characterized by scanning electron microscopy,infrared spectrum and ultraviolet visible spectrum.The high specific surface area and richness of amino groups of the RGO-PEI composite make it an excellent candidate for the detection of CTC due to the improved detection sensitivity and the stability of the molecularly imprinted film.Under optimized conditions,the sensor exhibited a wide linear range of(5.0×10^(-7))-(1.0×10^(-4))mol/L with a detection limit of 1.67×10^(-7)mol/L(S/N=3).Additionally,there was little response to interfering substances such as kanamycin,oxytetracycline and doxycycline hydrochloride.The proposed method was successfully used for the detection of CTC in real sample with the recoveries of 102.7%-104.7%,showing a simple and efficient electrochemical method in practical application.