运动应激对衰老大鼠神经免疫功能及HPA轴皮质酮分泌的影响

Effects of exercise stress on neuroimmune function and corticosterone secretion in HPA axis of aging rats

目的 探讨运动应激对衰老大鼠神经免疫功能及HPA轴皮质酮分泌效果与机制。方法 32只6周龄SPF级雄性Sprange-Dawley大鼠随机分为4组:对照组、衰老组、运动组、衰老运动组。采用D-半乳糖腹腔注射建立衰老氧化应激模型;采用8周跑台运动建立运动应激模型;用TBARS法检测海马皮质MDA含量,采用酶联免疫吸附试验检测不同脑区T-SOD、5-羟色胺(5-HT)以及血清皮质酮水平;结果 与对照组相比衰老组大鼠海马MDA含量高,具有统计学意义(P<0.01),T-SOD含量显著低于对照组(P<0.01),而运动组与对照组比较无统计学意义;与对照组比较衰老组大鼠5-HT含量在海马和皮质不同区域均显著低下(P<0.05);衰老组和运动组大鼠血清皮质酮含量均高于对照组(P<0.05);结论 长期的良性刺激可增强神经抗氧化能力,减少大鼠神经免疫功能的损害,从而维持机体免疫稳态,预防肿瘤等疾病的发生。

Objective To investigate the effect and mechanism of exercise stress on neuroimmune function and corticosterone secretion in HPA axis in aging rats.Methods Thirty two six week old SPF grade male sprage Dawley rats were randomly divided into four groups:control group,aging group,exercise group and aging exercise group.The aging oxidative stress model was established by intraperitoneal injection of D-galactose;The exercise stress model was established by 8-week treadmill exercise;The content of MDA in hippocampal cortex was detected by TBARS method,and the levels of T-SOD,5-hydroxytryptamine(5-HT)and serum corticosterone in different brain regions were detected by enzyme-linked immunosorbent assay;Results compared with the control group,the content of MDA in hippocampus of aging group was significantly higher(P<0.01),and the content of T-SOD was significantly lower than that of the control group(P<0.01);Compared with the control group,the content of 5-HT in aging group was significantly lower in different regions of hippocampus and cortex(P<0.05);The content of serum corticosterone in aging group and exercise group was higher than that in control group(P<0.05);Conclusion long term benign stimulation can enhance the antioxidant capacity of nerve and reduce the damage of neuroimmune function in rats,so as to maintain the immune homeostasis and prevent the occurrence of tumor and other diseases.