两种SD大鼠酒精依赖自由饮模型的建立

Establishment of Two Kinds of Alcohol-dependent Free Drinking Models in Sprague-Dawley Rats

目的建立两种SD大鼠酒精依赖模型的自由饮模型,为酒精依赖的动物实验提供实验模型依据。方法以30只雄性SD大鼠为研究对象,使用随机数表法分为对照组、10%双瓶自由连续性饮酒模型组和20%双瓶自由间歇性饮酒模型组,每组10只。在构建模型28 d后,进行行为学测试(高架十字迷宫、水迷宫)和酒精戒断综合征评分。结果3组大鼠在实验中体质量变化经比较,差异无统计学意义(P>0.05);2组模型组大鼠酒精摄入量逐渐增加到达平台期,饮水量伴随饮酒量的增多而降低到稳定,酒精偏好增加到稳定;高架十字迷宫检测中,2组模型组开放臂进入次数和开放臂滞留时间较对照组明显下降,差异有统计学意义(P<0.05);在水迷宫检测中,2组模型组记忆能力较对照组降低,差异有统计学意义(P<0.05);在酒精戒断评分检测中,2组模型组24 h戒断评分较对照组升高,差异有统计学意义(P<0.05)。结论两种酒精依赖的建模方法都可以用来开展相关酒精依赖动物实验研究。

Objective To establish two kinds of free drinking models of SD rats with alcohol dependence,and to provide experimental basis for animal experiment of alcohol dependence.Methods SD-rats(n=30,male)were randomly divided into control group(n=10),10%double-bottle model group(n=10)and 20%double-bottle model group(n=10),after 28 days of modeling,the behavioral tests(elevated plus maze,morris water maze)and the scores of ethanol withdrawal syndrome were performed.Results There was no signifi cant diff erence in body weight among the three groups(P>0.05),alcohol intake in the two groups reached the plateau stage,water intake decreased with the increase of alcohol intake,and alcohol preference increased to the stability(P<0.05).In the elevated plus maze test,the times of open arm entry and the retention time of open arm in the model group were signifi cantly lower than in the control group(P<0.05),and in the morris water maze test,the memory ability in the model group was lower than in the control group(P<0.05).The 24-hour abstinence score of the model group was higher than that of the control group(P<0.05).Conclusion Both of the two methods can be used to carry out the experimental study of alcohol dependence in animals.