玉米11个ZmbZIP基因的鉴定及表达特征分析

The identification of 11 ZmbZIPs of maize and their expression pattern analysis

以玉米自交系郑58为试验材料,利用生物信息学方法和qPCR技术,设置200 mmol/L NaCl、20%PEG6000、4℃低温和硝态氮或铵态氮缺乏等胁迫试验,对11个ZmbZIP基因进行生物信息学分析,探测这些基因应答低温、干旱、盐害、缺氮等胁迫时的响应表达模式。系统进化树分析结果表明,11个ZmbZIP基因可以细分为2个亚组;qPCR分析结果表明,不同亚组的ZmbZIP基因在玉米不同组织中呈现不同的表达特征,同一亚组的基因呈现类似的表达模式,反映了ZmbZIP基因在玉米生长发育进程中生物学功能的保守性和分化性。人为模拟盐、干旱、低温和氮缺乏等胁迫的结果表明,ZmbZIP基因受到各种逆境因子的广泛调控,亚组I中的ZmbZIP1、ZmbZIP6和ZmbZIP13的表达量同时受NaCl溶液胁迫诱导而上调(相对表达量上调2倍),受PEG6000胁迫抑制下调;亚组II中的ZmbZIP38、ZmbZIP77、ZmbZIP112和ZmbZIP124同时受盐、PEG6000和低温胁迫诱导上调,硝态氮缺乏胁迫抑制ZmbZIP1、ZmbZIP6和ZmbZIP134基因表达,而铵态氮缺乏胁迫下,这3个基因表达明显上调,推测这些基因广泛参与应答逆境胁迫响应途径。

Using maize inbreed line Zheng58 for experimental material,bioinformatic and expression pattern analysis of 11 ZmbZIP genes were performed at the experimental conditions of 200 mmol/L NaCl,20%PEG6000,4℃and nitrate or ammonium deficiency stresses.The phylogenetic tree analysis showed that 11 ZmbZIPs could be subdivided into 2 subgroups.RT-PCR results revealed that different expression patten of ZmbZIP were detected in different tissues,indicating different biological roles in plant growth and development.Under simulated salt,drought,low temperature and nitrogen stress,ZmbZIP were widely regulated,ZmbZIP1,ZmbZIP6 and ZmbZIP13 of subgroup I were obviously upregulated by salt by two fold,and inhibited by PEG6000,while ZmbZIP38,ZmbZIP77,ZmbZIP112 and ZmbZIP124 of subgroup II were upregulated by salt,PEG6000 and low temperature stresses.And,ZmbZIP1,ZmbZIP6 and ZmbZIP134 were obviously downregulated by nitrate deficient stress,while upregulated by ammonium deficient stress,indicating different biological function roles of these genes in responsive to various stresses.