黑龙江省马铃薯黑痣病菌融合群类型分析

Identity of the Anastomosis Group of Potato Black Scurf in Heilongjiang Province

为明确黑龙江省马铃薯黑痣病菌融合群类型和分布情况,采用ITS序列分析法分析了2012~2021年采自黑龙江省10个马铃薯种植区的482份马铃薯黑痣病菌株的融合群类型及频率分布。试验共鉴定出5种融合群,其中344个菌株属于AG-3,出现频率为71.37%;70个菌株属于AG-5,出现频率为14.52%;32个菌株属于AG-2,出现频率为6.64%;34个菌株属于AG-4,出现频率为7.05%;2个菌株属于AG-1,出现频率为0.42%。在AG-2和AG-4融合群中分别鉴定出AG-2-1、AG-2-2ⅢB和AG-2-2Ⅳ以及AG-4-HGI和AG-4-HGII五个融合亚群,出现频率依次为50.00%、6.25%、43.75%和70.59%、29.41%。结合黑龙江省马铃薯种植区域和融合群类型分析,表明AG-3为优势致病融合群,在全省马铃薯主产区均有分布,同时也反映出黑龙江省马铃薯黑痣病菌融合群类型较为复杂。因此,加强对黑痣病菌融合群类型变化的监测,对更好地进行马铃薯黑痣病防控具有重要意义。

Four hundred and eighty-two samples of black scurf isolates collected from 10 different potato growing regions of Heilongjiang Province in 2012 to 2021 were detected by ITS sequence analysis method in order to clarify the type and distribution of anastomosis group of black scurf in Heilongjiang Province. Five anastomosis groups were identified, of which 344 isolates belong to AG-3, with a frequency of 71.37%;70 isolates belong to AG-5, with a frequency of 14.52%;32 isolates belong to AG-2, with a frequency of 6.64%;34 isolates belong to AG-4, with a frequency of 7.05%;and two isolates belong to AG-1, with a frequency of 0.42%. In AG-2 and AG-4 anastomosis groups,five anastomosis subgroups, AG-2-1, AG-2-2ⅢB and AG-2-2Ⅳ, and AG-4-HGI and AG-4-HGII, were identified, and the occurrence frequency was 50.00%, 6.25% and 43.75%, and 70.59% and 29.41%, respectively. Combining the potato planting regions and anastomosis group analysis, AG-3 was considered the dominant pathogenic anastomosis group,which was distributed in the main potato producing regions of Heilongjiang Province. And it also reflects that the anastomosis group is relatively complex. Therefore, it is of great significance to strengthen the monitoring on the anastomosis group, which would be better for the control of black scurf.