生血方的鉴别与含量测定

Identification and Content Determination of Shengxue Fang

目的:建立生血方的鉴别和含量测定。方法:采用薄层色谱法(TLC)对生血方中黄芪、女贞子和当归进行定性鉴别;采用高效液相色谱法(HPLC-ELSD)对生血方中芒柄花素和黄芪甲苷进行含量测定,流动相为乙腈-水,流速1 mL·min^(-1);使用蒸发光散射检测器,载气气压0.5 MPa,漂移管温度为100℃。结果:在TLC图谱中,斑点清晰,分离度良好,阴性样品无干扰。方中2个成分(芒柄花素和黄芪甲苷)完全分离,峰面积的对数与进样量的对数呈良好的线性关系(r=0.999,0.9994)。3批样品中黄芪甲苷和芒柄花素的含量分别在0.6411~0.6735、0.4808~0.5008 mg·g^(-1)。结论:经方法学验证,该方法可用于生血方的质量控制。

Objective:To establish a method for identification and content determination for Shengxue Fang.Methods:Astragali Radix,Ligustrum Lucidum and Angelicae Sinensis Radix were identified by TLC qualitatively.The contents of formononetin and astragaloside Ⅳ were determined by HPLC-ELSD.The mobile phase of acetonitrile and water was eluted at 1.0 mL·min^(-1).The detector was ELSD,of which the drift tube temperature was maintained at 100℃,and the pressure of nebulizing gas was 0.5 MPa.Results:The TLC spots were clear and the resoluion was good without the interference of negative samples.The HPLC-ELSD results showed that the formononetin and astragaloside Ⅳ were completely separated;the regression equation showed good liner relationship between the logarithm of peak areas and the logarithm of contents of each compound(r=0.999,0.9994).The contents of formononetin and astragaloside Ⅳ in 3 Shengxue Fang samples were 0.6411-0.6735 mg·g^(-1) and 0.4808-0.5008 mg·g^(-1),respectively.Conclusion:The established method is validated by methodology,and is suitable for the quality control of Shengxue Fang.