小麦矮腥黑粉菌效应蛋白g11335的生物信息学分析、亚细胞定位及毒性验证

Bioinformatics Analysis,Subcellular Localization and Toxicity Verification of Effector g11335 in Tilletia contraversa Kühn

本研究重点分析小麦矮腥黑粉菌的g11335基因编码效应蛋白的生物学功能。效应蛋白g11335为GSDH家族的效应蛋白,该家族成员有影响植物体的可能性。运用多种生物信息学数据库,解析效应蛋白g11335的理化性质、保守结构域、跨膜区、亲疏水性以及信号肽,并且构建了g11335-pBin表达载体进行亚细胞定位分析。结果表明,g11335基因全长1389 bp,共编码462个氨基酸。该效应蛋白的亚细胞定位结果表明,该蛋白质定位在细胞膜上。烟草毒性验证表明该效应蛋白无毒性。

This research focused on analyzing the biological function of effector protein encoded by g11335 gene of Tilletia contraversa Kühn.Effector g11335 was an effector protein of GSDH family which may affect the plant healthy.The physicochemical properties,conserved domain,transmembrane domain,hydrophilicity and hydrophobicity of effector g11335 were analyzed using a variety of bioinformatics databases,and the expression vector g11335-pBin was constructed for subcellular localization analysis.The results showed that g11335 gene was 1389 bp in length and encoded 462 amino acids.Subcellular localization of the effector protein showed that the protein was localized on the cell membrane.Tobacco toxicity test revealed that the effector protein was not toxic.