烟草根际高效解磷菌的筛选鉴定及促生作用研究

Screening and Identification of High-efficiency Phosphate Solubilizing Bacteria in Tobacco Rhizosphere and Its Growth-promoting Effects

为获得优质的解磷促生菌种资源,助力农业生产减肥增效,对山东省高密市方市乡烟草根际土壤解磷菌进行筛选,将筛选得到的高效解磷菌3P29进行分子生物学鉴定,并研究其解磷性能及促生能力。菌株3P29鉴定为皮特不动杆菌,其对卵磷脂的转化量为13.38 mg/L,磷酸钙的转化量为19.83 mg/L,具有高效分解有机磷及无机磷的功能。烟草盆栽试验表明,烟草主根变长且有更多侧根产生,从而增强根对营养元素的吸收,烟草叶片全氮磷钾含量在无磷营养液条件下提高了71%、49%和134%,可为解磷菌肥的研发与利用提供优良菌株资源。

In order to obtain high-quality phosphate-solubilizing bacterial resources and enhance agricultural production efficiency,we screened phosphorus-solubilizing bacteria from the tobacco rhizosphere soil of Fangshi township,Gaomi city,Shandong province,among which the highly efficient phosphate-solubilizing bacterium 3P29 was identified by molecular biology,then the ability of phosphate-solubilizing and growth-promoting effect were further studied.The strain 3P29 was identified as Acinetobacter pittobacter and the transformation amount of lecithin and calcium phosphate were 13.38 mg/L and 19.83 mg/L,respectively,indicating that the strain 3P29 efficiently decomposed organic and inorganic phosphorus.Tobacco pot experiment showed that the primary root of tobacco became longer and more lateral roots were produced,thereby enhancing the absorption of nutrient elements by the roots.The total nitrogen,phosphorus and potassium content of tobacco leaves increased by 71%,49%and 134%under the conditions of phosphorus-free nutrient solution,which may provide superior strain resources for the development and utilization of phosphate solubilizing bacterial fertilizers.