不同日龄鸭肌肉组织多胺代谢和肌肉发育相关基因表达的规律

Expressions of Genes Related to Polyamine Metabolism and Muscle Development in Muscle Tissues of Duck at Different Ages

为研究并阐明鸭肌肉组织多胺代谢和肌肉发育相关基因表达的变化规律,利用高效液相色谱和实时荧光定量检测了0-120日龄鸭胸肌和腿肌组织中多胺含量、多胺代谢和肌肉发育相关基因表达量,并分析了多胺代谢和肌肉发育相关基因表达量的相关性。结果表明,0日龄鸭胸肌和腿肌组织中腐胺、亚精胺和精胺含量最高,胸肌和腿肌组织中多胺合成代谢相关基因分别呈一定规律变化;胸肌组织中,MyoD1和Myf5基因在0日龄时表达量最高,MyoG和IGF-1基因表达量在30日龄时最高,MSTN在90日龄时表达最高,且MyoD1和Myf5基因与SPMS、SPDS、SSAT、SMO基因呈显著的正相关;腿肌中,ODC与MyoD1和MSTN基因表达量呈显著的正相关。上述研究结果提示,鸭肌肉组织中多胺含量、多胺代谢和肌肉发育相关基因表达量的变化规律具有一定相似性,可为多胺调控鸭肌肉发育作用机制的研究提供参考。

In order to study and clarify the expression changes of genes related to polyamine metabolism and muscle development in ducks’muscle tissue,high performance liquid chromatography and RT-qPCR method were used to detect the polyamine contents and the expressions of polyamine metabolism and muscle development-related genes in the breast and leg muscle tissues during duck postnatal stage on day 0 to 120,and then to analyze the correlation between the expressions of muscle development-related gene and polyamine metabolism.Results showed that the contents of putrescine,spermidine and spermine in the duck breast and leg muscle at 0 days were the highest,and the polyamine metabolism genes in the breast and leg muscle tissue showed regular changes,respectively.In pectoral muscle tissue,the expressions of MyoD1 and Myf5 were the highest in 0 days,MyoG and IGF-1 were the highest at 30 days,and MSTN had the highest expression at 90 days,and MyoD1 and Myf5 genes were significantly and positively correlated with SPMS,SPDS,SSAT,and SMO genes.In leg muscle,ODC was significantly and positively correlated with MyoD1 and MSTN gene.The above results suggest that the changes in polyamine content,polyamine metabolism and muscle development-related gene expression in duck muscle tissues present a certain similarity,so as to provide the reference for studying polyamine regulating duck muscle development action mechanisms.