抗稻瘟病基因Pigm组合标记的开发及应用

Development and Application of the Combinatorial Marker for the Rice Blast Resistance Gene Pigm

Pigm是水稻抗稻瘟病基因之一,抗性广且持久。针对Pigm基因,已开发多种分子标记,但检测特异性或辨识度尚存不足。通过NCBI对7个Pigm标记进行序列比对分析,包括Pigm-4、T9E4、DG-2、M80410、R060939、GMR-3和T-Pigm,并对40个水稻品种开展检测验证。根据序列分析与检测结果开发了一种组合标记,应用于镇糯19×空育131(Pigm)分子标记辅助选择育种。序列分析结果表明,M80410标记特异性强,但不能区分杂合基因型;Pigm-4、T-Pigm和T9E4等标记能区分杂合基因型,但特异性略有不足。结合水稻品种检测结果,筛选了Pigm-4与M80410作为组合标记,并用于辅助育种,选育了糯稻品系NK29,具有较好稻瘟病抗性。组合标记能快速准确地鉴定Pigm 的纯合显性、纯合隐性和杂合基因型,为Pigm分子育种奠定基础。

Pigm is one of the rice blast resistance genes,with broad-spectrum and lasting resistance.A variety of molecular markers have been developed for Pigm gene,but the detection specificity or recognition is still insufficient.Seven Pigm markers were analyzed by NCBI,including Pigm-4,T9E4,DG-2,M80410,R060939,GMR-3 and T-Pigm,and total 40 rice varieties species were detected and verified.According to the sequence analysis and detection results,a combinatorial marker was developed and applied to Zhennuo 19 × Kongyu 131(Pigm)molecular marker-assisted selection.Sequence analysis showed that M80410 marker had strong specificity,but could not distinguish heterozygous genotypes;Pigm-4,T-Pigm and T9E4 etc.markers distinguished heterozygous genotypes,but their specificity was slightly insufficient.Combined with the detection results of rice varieties,Pigm-4 and M80410 were selected as combinatorial marker and used in assisted breeding.The glutinous rice line NK29 was selected,which had good resistance to rice blast.Combinatorial marker can quickly and accurately identify the homozygous dominant,homozygous recessive and heterozygous genotypes of Pigm,which lays a foundation for Pigm molecular breeding.