基于毛细管电泳的双酚与血清白蛋白行为成像

Rapid evaluation of the interaction between bisphenol E and serum albumin by capillary electrophoresis

目的 建立双酚E(BPE)与血清白蛋白相互作用快速评价方法,为双酚类化合物毒理效应研究提供有益参考。方法 使用毛细管电泳仪,应用外标Hummel-Dreyer(HD)法,测定了体外环境中BPE与血清白蛋白相互作用电泳图。采用非线性方程、Scatchard方程和Klotz方程三种函数形式分析实验数据,获得体系的相互作用参数。结果 三种不同函数形式所得到的拟合参数具有较高的一致性。BPE与血清白蛋白结合比约为2,相互作用参数表明牛血清白蛋白及人血清白蛋白的结合反应存在相似的结合位点数,且结合稳定。不同白蛋白与BPE的相互作用相似,均为非共价相互作用。结论 外标HD法作为一种简便有效的方法可以快速评价蛋白质-污染物相互作用相关问题。BPE与不同血清白蛋白具有相似的温和相互作用,这提示双酚类化合物在人体中和牛中可能具有类似的污染分布规律。

Objective The interaction between bisphenol E(BPE)and serum albumin was analyzed to provide a useful referrence for the rapid evaluation of the toxicological effect of bisphenols.Methods Electropherograms of serum albumin with BPE under physiological conditions in vitro were determined using external calibration Hummel-Dreyer(HD)method by capillary electrophoresis. To obtain the interaction parameters,the experimental data were analyzed by three functional forms:Nonlinear equation,Scatchard equation and Klotz equation.Results Three form functions were fitted with the interaction parameters. The binding ratio of BPE and serum albumin was about 2. The interaction parameters showed that two kinds of serum albumin had the same number of binding sites,and the binding between different albumin and BPE were both non-covalent interactions.Conclusion As a simple and effective method,external calibration HD method could be used to analyze the protein pollutant interaction in the food safety field. BPE showed similar moderate interaction with different serum albumin,which suggested that bisphenol compounds had similar pollution distribution regularity in human body and cattle.