分子印迹技术在致病菌检测中的应用进展

Recent advances of molecular imprinted technology in the detection of pathogenic bacteria

分子印迹技术是一种模拟酶-底物或抗体-抗原之间的相互作用,以目标物为模板,合成具有专一识别性能的人工受体的技术。前期研究对象多为农药、兽药等有机小分子及蛋白质类生物大分子。近年来,研究已拓展到超大“分子”——细胞,特别是细菌印迹在致病菌快速检测领域应用前景广阔。本文介绍了分子印迹技术的原理、细菌印迹模板的选择及印迹聚合物的制备方法,并总结了分子印迹技术在致病菌检测中的应用,并对其应用前景做出了展望。

Molecular imprinting technology is a kind of technology that simulates the interaction between enzyme-substrate or antibody-antigen and synthesizes artificial receptors with specific recognition performance using target as template. In the early stage,the research objects were mostly small organic molecules including pesticides and veterinary drugs,and biological macromolecules including proteins. In recent years,researches have been extended to super-large "molecules"-cells,especially bacterial imprinting with a broad application prospect in the field of rapid detection of pathogenic bacteria. This paper introduces the principle of molecular imprinting technology,the selection of bacterial imprinting template and the preparation method of imprinted polymers. The application of molecular imprinting technology in the detection of pathogenic bacteria is summarized. Finally,the application prospect of molecular imprinting technology in the detection of pathogenic bacteria is discussed.