水稻纹枯病菌谷氨酸合成酶RsGLT1基因的克隆及生物信息学分析

Cloning and Bioinformatics Analysis of Glutamate Synthase RsGLT1 Gene of Rice Sheath Blight

谷氨酸是生物体内重要的氨基酸,谷氨酸的体内平衡对维持生物的正常生长和代谢非常重要。同时,谷氨酸的稳态也影响病原真菌对宿主的附着、侵染等过程,是其致病力的重要影响因子。谷氨酸合成酶,是谷氨酸合成途径中的重要影响因子。为了对水稻纹枯病菌中的GLT1基因编码蛋白进行结构和分子功能分析,本研究从水稻纹枯病菌中克隆获得了一个谷氨酸合成酶相关基因RsGLT1(NADPH-dependent glutamate synthase,GLT1),并运用多种在线生物信息学分析工具,对该基因的同源性、理化性质、亲/疏水性、信号肽、功能域等进行了分析。结果显示,RsGLT1基因CDS长6471 bp,编码2156个氨基酸;该基因具有典型的GltB和GltD两个结构域,是一个非跨膜的亲水蛋白,不具有信号肽结构。本研究为研究水稻纹枯病菌谷氨酸合成调控途径及RsGLT1基因对水稻纹枯病菌致病力的影响奠定了理论基础。

Glutamate is one of the most important amino acids in vivo.It plays a vital role in regular growth and metabolism.Glutamate homeostasis is a key factor in pathogenic fungi which affects attachment and infection.The glutamate synthase is an important influencing factor in the pathway of glutamate synthesis.In order to analyze the structure and molecular function of GLT1 gene encoding protein in Rhizoctonia solani Kühn,a glutamate synthase gene RsGLT1 had being identified and cloned in Rhizoctonia solani Kühn,the homology was predicted by using a series of online bioinformatics tools,physicochemical properties,hydrophilic/hydrophobic properties,signal peptides and functional domains of the gene.The results showed that the CDS sequence of RsGLT1 gene was 6471 bp and the number of amino acids was 2156.The bioinformatics analysis showed that RsGLT1 protein has the typical GltB and GltD domain.It was hydrophilic but without transmembrane structure and signal peptide.This study would lay a basis for understanding the regulatory pathway of glutamate synthesis and the study of the function of GLT1 in the Rhizoctonia solani.