拟南芥及玉米过氧化氢酶基因家族生物信息学分析

Bioinformatics Analysis of Catalase Gene Family of Arabidopsis and Maize

过氧化氢酶(catalase,CAT)是一种强抗氧化物酶,又称触酶,在植物发育过程和植物非生物胁迫响应中起着关键的作用。过氧化氢酶的作用在植物中有很多研究,但是对编码该酶的基因(CATs)的研究较少。本研究利用NCBI和Phytozome数据库共鉴定出3个玉米ZmCATs和3个拟南芥AtCATs基因。并对其基因家族进行了蛋白特性、进化关系、基因结构、蛋白质二级和三级结构,以及不同发育阶段表达和非生物胁迫下的表达分析。结果显示,CATs家族在两个物种中具有一定的相似性,ZmCATs和AtCATs基因所编码的蛋白质均为疏水性蛋白。根据系统发育树分析将CATs基因家族分为两个亚家族,Zm CATs与AtCATs都包含2个保守结构域;同时,ZmCATs和At CATs蛋白二级结构均以α-螺旋和无规则卷曲为主要结构。通过对ZmCATs在不同生长发育阶段表达量的聚类分析,发现ZmCAT1,ZmCAT2,ZmCAT3在玉米生长发育阶段有较高表达量,其中ZmCAT3在生长发育阶段与其他基因相比表达量较高。在非生物胁迫下,ZmCATs基因的表达受温度胁迫影响较为明显。本研究结果为探究玉米等作物CAT的功能提供一定的理论依据。

Catalase(CAT)is a strong antioxidant enzyme,also known as catalase,which plays a key role in plant development and plant abiotic stress response.However,there are few studies on the genes encoding the enzyme(CATs).In this study,three maize ZmCATs and three Arabidopsis AtCATs genes were identified by screening NCBI and phytozome databases.The protein characteristics,evolutionary relationship,gene structure,protein secondary and tertiary structure,gene expression at different developmental stages or under abiotic stress treatment were analyzed.The results showed that there were some similarities between the two species.The proteins encoded by ZmCATs and AtCATs genes were hydrophobic.According to phylogenetic tree analysis,CATs gene family was divided into two subfamilies.ZmCATs and AtCATs both contain two conserved domains,and the secondary structures of ZmCATs and AtCATs protein are mainly α-helix and random coils.The cluster analysis of ZmCATs expression in different growth and development stages showed that ZmCAT1,ZmCAT2 and ZmCAT3 were highly expressed in maize growth and development stage,and ZmCAT3 was more expressed than other genes in growth and development stages.Under abiotic stress,the expression of ZmCATs gene was significantly affected by temperature stress.The results of this study provide a theoretical basis for exploring the function of CAT in maize and other crops.