毛竹木犀草素-6-C-新橙皮苷通过调节IRF4PTG糖原途径促进力竭运动小鼠的糖原合成

Mao Bamboo LN Promotes Glycogen Synthesis in Exhaustive Exercise Mice by Regulating the IRF4PTG Glycogen Pathway

本研究旨在揭示从毛竹叶中分离的木犀草素-6-C-新橙皮苷(LN)对力竭运动诱导的骨骼肌损伤和疲劳的影响和机制。将雄性昆明小鼠随机分成5组,各处理组小鼠连续给药4周后进行强迫游泳实验。运动后检测小鼠血清疲劳指标、糖原、骨骼肌氧化应激和炎症指标水平。通过RT-qPCR或Western blot检测骨骼肌中导向糖原蛋白(PTG)、干扰素调节因子4(IRF4)和糖原合成酶(GS)的表达。结果显示,与Swim-Ctrl组相比,Swim-LN-L组、Swim-LN-M组和Swim-LN-H组的CK、BUN和LDH水平降低(P<0.05)。与Swim-Ctrl组相比,Swim-LN-L组、Swim-LN-M组和Swim-LN-H组的MDA水平降低,而SOD和CAT水平升高(P<0.05)。与Swim-Ctrl组相比,Swim-LN-L组、Swim-LN-M组和Swim-LN-H组的IL-1β、TNF-α和IL-6水平降低(P<0.05)。与Swim-Ctrl组相比,Swim-LN-L组、Swim-LN-M组和Swim-LN-H组的肝糖原和肌糖原含量升高(P<0.05)。与Swim-Ctrl组相比,Swim-LN-L组、Swim-LN-M组和Swim-LN-H组小鼠骨骼肌组织中IRF4的表达水平降低,而PTG和GS升高(P<0.05)。本研究表明从毛竹叶分离的木犀草素-6-C-新橙皮苷可以提高力竭游泳小鼠的抗疲劳能力,减轻骨骼肌损伤,抑制氧化应激和炎症。LN通过调控力竭游泳小鼠骨骼肌中IRF4/PTG/糖原途径来促进糖原合成。

The purpose of this study was to reveal the effect and mechanism of luteolin-6-c-neohesperidin(LN)isolated from Mao Bamboo leaves(Phyllostachys edulis(Carriere)J.Houzeau)on skeletal muscle injury and fatigue induced by exhaustive exercise.Male Kunming mice were randomly divided into 5 groups,and the mice in each treatment group were subjected to forced swimming test after continuous administration for 4 weeks.After exercise,serum fatigue index,glycogen,skeletal muscle oxidative stress and inflammatory index levels were measured.RT q PCR or Western blot was used to detect the expression of guided glycogen protein(PTG),interferon regulatory factor 4(IRF4)and glycogen synthase(GS)in skeletal muscle.The results showed that compared with the SwimCtrl group,the levels of CK,BUN and LDH in the Swim-LN-L group,Swim-LN-M group and Swim-LN-H group were lower(P<0.05).Compared with the Swim-Ctrl group,the MDA level of the Swim-LN-L group,the Swim-LN-M group and the Swim-LN-H group decreased,while the SOD and CAT levels increased(P<0.05).Compared with the Swim-Ctrl group,the levels of IL-1β,TNF-α and IL-6 in the Swim-LN-L group,Swim-LN-M group and Swim-LN-H group were lower(P<0.05).Compared with the Swim-Ctrl group,the contents of liver glycogen and muscle glycogen in the Swim-LN-L group,Swim-LN-M group and Swim-LN-H group increased(P<0.05).Compared with the Swim-Ctrl group,the expression level of IRF4 in the skeletal muscle tissue of mice in the Swim-LN-L group,Swim-LN-M group and Swim-LN-H group decreased,while PTG and GS increased(P<0.05).This study shows that LN can improve the fatigue resistance of exhausted swimming mice,reduce skeletal muscle damage,and inhibit oxidative stress and inflammation.LN promotes glycogen synthesis by regulating the IRF4/PTG/glycogen pathway in the skeletal muscle of exhausted swimming mice.