蓝刺头中总黄酮的含量测定

Determination of total flavonoids in Echinops Latifolius Tausch

目的 建立蓝刺头总黄酮含量的测定方法。方法 用60%乙醇作为溶剂超声提取蓝刺头总黄酮,以芹菜苷为标准品,5%三氯化铝溶液显色,按照紫外-可见分光光度法(中华人民共和国药典2020年版四部通则0401)在275 nm波长处测定供试品溶液的吸光度,计算蓝刺头中总黄酮的含量。结果 芹菜苷质量浓度在3.28~32.80μg/ml范围内与吸光度线性关系良好,回归方程为Y=0.0357X-0.0065(r=0.9999);该测定法经考察表明具有良好的精密度、重复性、稳定性和准确度,平均加样回收率为100.20%,RSD为0.923%(n=6);3批蓝刺头药材的总黄酮平均含量为2.30%。结论 本测定法基于黄酮类化合物结构与光吸收特性关系而建立,重现性好,准确度高,适用蓝刺头中总黄酮的含量测定。

Objective To establish a method for the determination of total flavonoids in Echinops Latifolius Tausch. Methods The total flavonoids of Echinops Latifolius Tausch were extracted by ultrasonic with 60% ethanol. Apigenin was used as the standard substance, and 5% aluminum trichloride solution was used as chromogenic agent. The absorbance of the test solution was determined at 275 nm by ultraviolet visible spectrophotometry according to the general rule 0401 of Part Ⅳ of Chinese Pharmacopoeia 2020 edition. And the content of total flavonoids in Echinops Latifolius Tausch was calculated. Results The mass concentration of apigenin has a good linear relationship with absorbance in the range of 3.28-32.80 μg/ml, and the regression equation was Y=0.0357X-0.0065(r=0.9999). The results showed that the method had good precision,repeatability, stability and accuracy. The average recovery was 100.20% and RSD was 0.923%(n=6). The average content of total flavonoids in three batches of Echinops Latifolius Tausch was 2.30%. Conclusion This method is based on relationship between the structure of flavonoids and light absorption characteristics. It is reproducible, accurate and suitable for the determination of total flavonoids in Echinops Latifolius Tausch.