大鼠神经病理性痛关键基因的筛选

Identification of key genes for neuropathic pain in rats

目的筛选大鼠神经病理性痛的关键基因。方法在美国生物技术信息中心的基因表达数据库中,下载大鼠神经病理性痛脊髓组织的基因组数据(GSE18803),确定神经病理性痛相关的差异表达基因,进一步分析蛋白质相互作用网络得到关键基因。通过Tabula Muris数据库对关键基因进行免疫炎症单细胞分布和表达情况的分析。结果蛋白质相互作用网络得到10个关键基因,包括Tyrobp、Clec4a3、C1qc、Ptprc、Laptm5、Csf1r、C1qa、C1qb、Fcgr3a和Cd53。Cd53、Laptm5和Ptprc主要在巨噬细胞、B细胞、NK细胞、单核细胞和粒细胞中存在表达;Clec4a3和Csf1r主要在单核细胞中表达,Fcgr3a主要在单核细胞和粒细胞中存在表达;Tyrobp主要在巨噬细胞、单核细胞、粒细胞和多能祖细胞中存在表达。结论通过生物信息学方法筛选出大鼠神经病理性痛相关的10个关键基因,并获得了其在脊髓免疫炎症细胞中的分布和表达情况。

Objective To identify the key genes for neuropathic pain in rats.Methods The genomic data of spinal cord tissues of rats(GSE18803)were downloaded from the Gene Expression Database at the American Center for Biotechnology Information to identify differentially expressed genes associated with neuropathic pain,and key genes were obtained by further analysis of the protein-protein interaction networks.Single-cell localization and expression of the key genes were analyzed by the Tabula Muris database.Results The protein-protein interaction networks identified 10 hub genes,including Tyrobp,Clec4a3,C1qc,Ptprc,Laptm5,Csf1r,C1qa,C1qb,Fcgr3a,Cd53.Cd53,Laptm5 and Ptprc were mainly expressed in macrophages,B cells,NK cells,monocytes and granulocytes.Clec4a3 and Csf1r were mainly expressed in monocytes,Fcgr3a in monocytes and granulocytes,and Tyrobp in macrophages,monocytes,granulocytes,and pluripotent progenitor cells.Conclusions Ten target genes associated with neuropathic pain are identified using bioinformatics,and their distribution and expression in immune inflammatory cells are obtained through comprehensive analysis.