恩施地区吸烟人群P450和GST基因多态性与肺癌易感性研究

Study on CYP450 and GST gene polymorphisms and lung cancer susceptibility in smoking population in Enshi area

目的研究细胞色素酶P450基因(CYP450)和谷胱甘肽S转移酶基因(GST)基因多态性与恩施地区吸烟人群非小细胞肺癌发病的相关性。方法收集2019年1月至2020年1月间在该院就诊并确诊为非小细胞肺癌患者和同期住院的肺良性疾病患者临床资料各70例,使用聚合酶链式反应(PCR)的方式扩增并测序患者CYP450和GST基因多态性并分析与肺癌的易感相关性。结果肺癌组和对照组c1/c1、c1/c2、c2/c2基因型的检出率分别为40.0%(28/70)、35.7%(25/70)、24.3%(17/70)和27.1%(19/70)、41.4%(29/70)、31.4%(22/70),以c2/c2基因型个体OR值为1,c1/c1个体肺癌OR为1.907(P<0.05)。肺癌组和对照组GST(+)和GST(-)基因型的检出率分别为37.1%(26/70)、62.9%(44/70)和51.4%(36/70)、48.6%(34/70),以GST(-)基因型个体OR值为1,GST(+)个体肺癌OR为0.546(P<0.05)。以c1/c2+c2/c2/GST(+)OR值为1,c1/c2+c2/c2/GST(-)、c1/c1/GST(-)、c1/c1/GST(-)的OR值分别为2.315,2.412,4.878(P<0.05)。结论CYP2E1和GSTM1可能参与了吸烟相关致癌物质的解毒过程。携带功能缺失的GSTM1(-)和CYP450 c1/c1基因型人群非小细胞肺癌的风险显著增高,同时携带两个危险基因型的吸烟人群非小细胞肺癌发生风险提高约5倍。

Objective To investigate the correlation between cytochrome P450 gene(CYP450)and glutathione transferase gene(GST)gene polymorphisms and the incidence of non-small cell lung cancer in smoking population in Enshi area.Methods The clinical data of 70 patients with non-small cell lung cancer and 70 patients with benign lung diseases who hospitalized in the hospital from January 2019 to January 2020 were collected,and the CYP450 and GST gene polymorphisms of the patients were amplified and sequenced by polymerase chain reaction(PCR)and their correlation with lung cancer susceptibility was analyzed.Results The detection rates of c1/c1,c1/c2 and c2/c2 genotypes in lung cancer group and control group were 40.0%(28/70),35.7%(25/70),24.3%(17/70)and 27.1%(19/70),41.4%(29/70)and 31.4%(22/70),respectively.When the OR value of c2/c2 genotype was taken as 1,OR value of c1/c1 genotype was 1.907(P<0.05).The detection rates of GST(+)and GST(-)genotypes in lung cancer group and control group were 37.1%(26/70),62.9%(44/70),51.4%(36/70)and 48.6%(34/70),respectively.When the OR value of GST(-)genotype was taken as 1,OR value of GST(+)genotype was 0.546(P<0.05).The OR value of c1/c2+c2/c2/GST(+)lung cancer was taken as 1,the OR values of c1/c2+c2/c2/GST(-),c1/c1/GST(-)and c1/c1/GST(-)were 2.315,2.412 and 4.878 respectively(P<0.05).Conclusion CYP2 E1 and GSTM1 might be involved in the detoxification process of smoking-related carcinogens.The risk of non-small cell lung cancer significantly increases in population with loss-of-function GSTM1(-)and CYP450 c1/c1 genotypes,and the risk of non-small cell lung cancer in smoking population with two dangerous genotypes at the same time increases by about 5 times.