毛柄金钱菌碳材料修饰电极测定过氧化氢

Determination of hydrogen peroxide by carbon material modified electrode derived from Flammulina velutiper

以毛柄金钱菌为碳前驱体制备了一种生物质多孔碳材料(BPC),并将其修饰到玻碳电极(GCE)表面,以此构筑了一种电化学传感器(BPC/GCE).采用扫描电镜(SEM)、X射线粉末衍射(XRD)、X射线光电子能谱(XPS)、拉曼(Raman)光谱和氮气吸附-脱附测试对BPC的形貌和结构进行了表征.运用循环伏安法(CV)、交流阻抗法(EIS)及电流-时间曲线研究了过氧化氢(H_(2)O_(2))在该传感器上的电化学行为.结果显示,基于BPC/GCE电极的H_(2)O_(2)传感器在15~300μmol/L范围内,H_(2)O_(2)的浓度与传感器信号呈线性关系,检出限为7.5μmol/L,且抗干扰能力强.利用所制备的H_(2)O_(2)传感器检测人尿中的H_(2)O_(2)显示,其加标回收率为95.9%~101.2%,相对标准偏差为2.8%~8.7%,因此该方法可用于人体液中H_(2)O_(2)的检测.

Biomass derived mesoporous carbon(BPC)was prepared by carbonization of Flammulina velutiper,and then modified on the surface of glassy carbon electrode(GCE)to construct electrochemical sensor.The morphology and structure of BPC were characterized by scanning electron microscopy(SEM),X-ray powder diffraction(XRD),X-ray photoelectron spectroscopy(XPS),Raman spectroscopy and Nadsorption and desorption method.The electrochemical behavior of H_(2)O_(2)on the sensor was studied by cyclic voltammetry(CV),AC impedance spectroscopy(EIS)and current time curve.In the range of 15-300μmol/L,the H_(2)O_(2)concentration was linear to the sensor signal,the detection limit is 7.5μmol/L,and the anti-interference ability is strong for the obtained H_(2)O_(2)sensor based on the BPC/GCE electrode.Furthemore,detection the H_(2)O_(2)in human urine display the recovery rate of H_(2)O_(2)was 95.9%-101.2%,and the relative standard deviation was 2.8%-8.7%.Thus,the method can use for the detection of H_(2)O_(2)in body fluids.