基于网络药理学探讨补肾祛风法代表药对“益智仁-防风”在糖尿病肾病治疗中的有效成分及作用机制

Exploration of the effective components and mechanism of Alpinia oxyphylla-Saposhnikovia Radix in the treatment of diabetic kidney disease based on network pharmacology

目的:借助网络药理学预测基于补肾祛风法的药对“益智仁-防风”治疗糖尿病肾病的关键化合物和有效成分的主要作用靶点及相关机制通路。方法:通过中药数据平台筛选出“益智仁-防风”有效成分,筛选有效靶点后,借助疾病靶基因数据库获取糖尿病肾病的作用靶点,通过维恩分析获得关键靶基因后利用基因富集分析预测相关信号通路。结果:利用中药数据平台筛选出“益智仁-防风”有效成分有324个,利用维恩分析图结合、交叉分析后预测其治疗糖尿病肾病关键靶基因有48个;“益智仁-防风”作用于糖尿病肾病的生物路径与NF-kappaB、classical MAP kinase、JNK and p38 MAP kinase、ERK5等信号通路关系密切。结论:基于补肾祛风法使用“益智仁-防风”治疗糖尿病肾病具有“多成分-多靶点-多通路”的作用特点,能够为进一步开展基于补肾祛风法使用“益智仁-防风”治疗糖尿病肾病的研究提供新的思路和方法。

Objective:Network pharmacology was used to predict the main targets and related mechanism pathways of the key compounds and active ingredients of the Alpinia oxyphylla-Saposhnikovia Radix based on the therapeutic strategy of tonifying kidney and dispelling pathogenic wind for the treatment of diabetic kidney disease(DKD).Methods:The effective components of Alpinia oxyphylla-Saposhnikovia Radix were screened through the data platform of Traditional Chinese Medicine(TCM).After screening effective targets,the disease target gene database was used to obtain targets for DKD,and then gene enrichment analysis to predict relevant signalling pathways after obtaining the key target genes through Venn analysis.Results:There were 324 active components of Alpinia oxyphylla-Saposhnikovia Radix through the data platform of TCM.In addition,Venn analysis showed that there were 48 key target genes for the treatment of DKD.The biological pathways for the action of Alpinia oxyphylla-Saposhnikovia Radix in DKD were closely related to many signaling pathways including NF-kappaB,classical MAP kinase,JNK and P38 MAP kinase,and ERK5.Conclusion:Treatment of DKD with Alpinia oxyphylla-Saposhnikovia Radix based on the therapeutic strategy of tonifying kidney and dispelling pathogenic wind has the characteristics of multi-components,multi-targets and multi-pathways,which could provide new ideas and methods for further researches on the treatment of DKD with Alpinia oxyphylla-Saposhnikovia Radix.