MicroRNA-27b在高血压脑出血大鼠脑组织中的表达及对星形胶质细胞生物学功能的影响

Expression of microRNA-27b in brain tissue of rats with hypertensive intracerebral hemorrhage and its effect on biological function of astrocytes

目的探讨microRNA-27b(miR-27b)在高血压脑出血(HICH)大鼠脑组织中的表达及对星形胶质细胞生物学功能的影响。方法选取15只健康WKY大鼠作为对照组,15只自发性高血压大鼠作为HICH组。HICH组通过自体血脑内注射法复制HICH大鼠模型,对照组进行假手术但不注射自体血。HE染色和TUNEL染色检测大鼠脑组织损伤和凋亡情况,实时荧光定量聚合酶链反应(qRT-PCR)检测miR-27b mRNA相对表达量。将星形胶质细胞分为NC组、miR-27b mimic组及miR-27b inhibitor组,分别转染miR-27b mimic和miR-27b inhibitor质粒,过表达和抑制miR-27b水平。qRT-PCR检测各组细胞miR-27b mRNA相对表达量,CCK-8法检测各组细胞增殖及凋亡情况。结果HICH组大鼠出血脑组织出现显著损伤,凋亡指数(35.91±3.24)高于对照组(2.75±0.36)(P<0.05);HICH组miR-27b mRNA相对表达量(3.45±0.48)高于对照组(1.00±0.12)(P<0.05);miR-27b mimic组的miR-27b mRNA相对表达量高于NC组,增殖活力低于NC组,凋亡率高于NC组(P<0.05)。miR-27b inhibitor组的miR-27b mRNA相对表达量低于NC组,增殖活力高于NC组,凋亡率低于NC组(P<0.05)。结论miR-27b mRNA相对表达量在HICH大鼠脑组织中显著升高,并抑制星形胶质细胞的增殖,促进星形胶质细胞凋亡。

Objective To explore the expression of microRNA(miR)-27b in the brain tissue of hypertensive intracerebral hemorrhage(HICH)rats and its effect on the biological functions of astrocytes.Methods Fifteen healthy WKY rats served as the control group.Fifteen spontaneously hypertensive rats served as the HICH group.Rats in the HICH group were injected with autologous blood into the brain to construct a HICH model.The control group underwent sham operation but did not inject autologous blood.The brain tissue damage and apoptosis were detected by HE staining and TUNEL staining.The expression of miR-27b was detected by RT-qPCR.Astrocytes were divided into NC,miR-27b mimic group,and miR-27b inhibitor group.According to the group,the miR-27b mimic or miR-27b inhibitor plasmids were transfected to overexpress or inhibit the level of miR-27b.The levels of miR-27b and the levels of cell proliferation and apoptosis in each group of cells were detected.Results Significant damage to the brain tissue of hemorrhage in the HICH group.The apoptosis index(35.91±3.24)and the expression level of miR-27b(3.45±0.48)in the HICH group were significantly higher than those in the control group(2.75±0.36,1.00±0.12)(P<0.05).The miR-27b level of the miR-27b mimic group was significantly higher than that of the NC group,the proliferation activity was significantly lower than that of the NC group,and the apoptosis rate was significantly higher than that of the NC group(P<0.05).The miR-27b level of the miR-27b inhibitor group was significantly lower than that of the NC group,the proliferation activity was significantly higher than that of the NC group,and the apoptosis rate was significantly lower than that of the NC group.Conclusion miRNA-27b is significantly increased in the brain tissue of HICH rats,inhibiting the proliferation of astrocytes and promoting their apoptosis.