升降通癃方中空栓对前列腺增生大鼠模型的影响

Comparison of curative effect of Shengjiangtonglong Prescription on anus and intragastric administration in benign prostatic hyperplasia rats

目的探讨升降通隆方中空栓对前列腺增生大鼠模型的影响。方法将70只雄性SD大鼠随机分为假手术对照组、模型组、非那雄胺阳性组、升降通癃方中空栓高、中、低剂量组、升降通隆方溶液灌胃组,每组10只,除假手术组外,其余各组均经去势后皮下注射丙酸睾丸酮复制良性前列腺增生(benign prostatic hyperplasia,BPH)大鼠模型,连续给药28天。计算前列腺指数,观察苏木精-伊红染色法(hematoxylin-eosin staining,HE)染色前列腺组织形态变化,采用酶联免疫法(enzyme linked immunosorbent assay,ELISA)测定血清和前列腺中睾酮(testosterone,T)、雌二醇(estradiol,E2)、血管内皮生长因子(vascular endothelial growth factor,VEGF)的水平。结果模型组较假手术组大鼠前列腺湿质量、指数明显上升(P<0.05),光镜下前列腺上皮细胞有显著的增生褶皱,模型复制成功;与模型组比,阳性组与高剂量组大鼠前列腺湿质量与指数均明显下降(P<0.05),中剂量组与灌胃组大鼠前列腺指数下降(P<0.05),各给药组大鼠前列腺和血清中T水平明显上升(P<0.05),E2与VEGF水平明显下降(P<0.05);与低剂量组比,高剂量组大鼠前列腺湿质量与指数均明显下降(P<0.05),高剂量组与中剂量组大鼠前列腺T水平明显上升(P<0.05),E2水平明显下降(P<0.05),高剂量组大鼠血清中T水平和VEGF水平明显上升(P<0.05);与灌胃组比,中剂量组大鼠前列腺湿质量与指数差异不明显(P>0.05),大鼠前列腺和血清中T与VEGF的水平差异影响不明显(P>0.05),中剂量组大鼠前列腺E2的水平明显下降(P<0.05)。结论升降通隆方中空栓纳肛直肠给药对去势大鼠前列腺增生具有良好的疗效,具有一定药效效应,升降通隆方中空栓高剂量药效更佳,且较灌胃给药治疗良性前列腺增生大鼠的药效相近,甚至更佳。

Objective To investigate the effects of Shengjiangtonglong Prescription hollow suppository on prostatic hyperplasia in rats.Methods 70 male SD rats were randomly divided into the control group,the model group,the positive finasteride group,the lift Long square hollow suppository high,medium and low dose group,lift tong lung lavage solution group,with10 rats in each group.,in addition to the control group,both groups after castration to duplicate the BPH model induced by subcutaneous injection of testosterone propionate,continuous dosing 28 days.The prostate index was calculated,the morphological changes of prostate tissues were observed by HE staining,and the levels of testosterone(T),estradiol(E2)and vascular endothelial growth factor(VEGF)in serum and prostate were determined by ELISA.Results Compared with the sham operation group,the wet weight and index of prostate in the model group were significantly increased(P>0.05).Compared with the model group,wet weight and index of prostate in the positive group and the high-dose group were significantly decreased(P<0.05),prostatic gland index in the medium-dose group and the gavage group was significantly decreased(P<0.05),T levels in prostate and serum were significantly increased(P<0.05),E2 and VEGF levels were significantly decreased(P<0.05).Compared with the low-dose group,the wet weight and index of prostate in the high-dose group were significantly decreased(P<0.05),the level of T in prostate in the high-dose group and the medium-dose group was significantly increased(P<0.05),the level of E2 was significantly decreased(P<0.05),and the serum LEVEL of T and VEGF in high-dose group were significantly increased(P<0.05).Compared with the gavage group,there was no significant difference in the wet weight and index of prostate in medium-dose group(P>0.05),and no significant difference in The levels of T and VEGF in prostate and serum(P>0.05),and the level of E2 in prostate in the medium-dose group was significantly decreased(P<0.05).Conclusion The analorectal administration of Shengjiangtonglong Prescription hollow suppository has good efficacy in the treatment of benign prostatic hyperplasia in emasculated rats,and has a certain pharmacodynamic effect.The high-dose efficacy of Shengjiangtonglong Prescription hollow suppository is better,and the efficacy of Shengjiangtonglong Prescription hollow suppository is similar to or even better than that of gavage in the treatment of benign prostatic hyperplasia in rats.