胶原蛋白肽联合大米肽促进皮肤健康改善功能的评价

Evaluation of collagen and rice compound peptides in improving skin care

探讨胶原蛋白肽(collagen peptides,CP)与大米肽(rice peptides,RP)对皮肤美容的促进作用。针对CP、RP及其复配物,采用体外方法考察了保湿功能、总抗氧化能力及对酪氨酸酶活性的抑制作用,进一步基于小鼠黑素瘤细胞(B16)评价了2种肽及其复配物在细胞内的抗氧化作用和抑制酪氨酸酶活性的作用。CP与RP均具有良好的体外保湿活性,并且CP及其与RP一定比例的复配物保湿活性优于同等条件下的5%甘油,2种肽均具有体外抗氧化功能,并能够抑制酪氨酸酶活性,且复配时能够协同增效。复配物能够清除B16细胞内的氧自由基,提高B16细胞内超氧化物歧化酶的活力,显著降低B16细胞内的酪氨酸酶活性,最佳复配比例为胶原蛋白肽∶大米肽=2∶1。以2∶1比例复配的CP与RP可能在保湿、美白、抗氧化等皮肤健康改善方面具有积极作用。

To investigate the promoting effects of collagen peptides(CP)and rice peptides(RP)on skin moisturizing,whitening and anti-oxidation.CP,RP and their complexes on skin care were investigated in vitro,with 5%glycerol as positive control,the water retention of the samples was investigated by weight loss method.Total antioxidant capacity was used to evaluate the antioxidant capacity of samples.The whitening activity of samples was evaluated by tyrosinase inhibition method.Based on the results of in vitro experiments,the proportion of cells was designed.The changes of superoxide dismutase(SOD)activity and reactive oxygen species(ROS)level in mouse melanoma cells(B16)were investigated to reflect the antioxidant capacity of the samples.The activity of tyrosinase in B16 cells was investigated to reflect the whitening ability of B16 cells.The results showed that both CP and RP showed good moisturizing activity in vitro,meanwhile,CP and its complex with RP in a certain proportion were superior to 5%glycerol under the same conditions.Both peptides had antioxidant function in vitro(ORAC method),and could inhibit tyrosinase activity,and synergistic synergistic effect was obtained when they were combined.The complex could scavenge ROS in B16 cells,improve the activity of SOD in B16 cells,and significantly reduce the activity of tyrosinase in B16 cells.The optimal complex ratio was CP∶RP=2∶1.Moreover,CP showed better antioxidant and moisturizing effects,while RP had better inhibitory effects on tyrosinase activity.The combined raw material could have synergistic effect in promoting moisture retention,anti-oxidation and inhibiting tyrosinase compared to each of them.The combination of collagen peptides and rice peptides in the ratio of 2:1 showed a stronger moisturizing effect than 5%glycerol in vitro,and it also showed a stronger tyrosinase inhibitory activity.In the cell experiment of mouse melanoma cell B16,it could clear the intracellular ROS,improve the activity of intracellular SOD,and significantly reduce the activity of tyrosinase.Collagen peptides and rice peptides at the ratio of 2:1 may play a positive role in skin health improvement,such as moisturizing,whitening and anti-oxidation.