麒麟菜残渣的亚硝酸钠吸附能力研究

Eucheuma sp.waste residue adsorption capacity of sodium nitrite

亚硝酸钠是食品添加剂中毒性最强的物质之一,不溶性膳食纤维(insoluble dietary fiber,IDF)具有很好的亚硝酸钠吸附效果。为了降低亚硝酸钠给人类造成的危害,该文研究了麒麟菜提取卡拉胶之后剩余残渣的化学组成,随后将麒麟菜残渣超微粉碎并测定了它们的水合特性和阳离子交换能力,在此基础上测定了麒麟菜残渣超微粉在体外模拟胃液下的亚硝酸钠吸附能力和亚硝胺合成阻断能力。结果显示,麒麟菜残渣的IDF含量高达90.1%,持水力和膨胀力最高分别达到(5.61±0.06)g/g和(5.67±0.30)mL/g,阳离子交换能力最高为(0.32±0.02)mmol NaOH/g;其在5 min内对亚硝酸钠的吸附率达到(70.0±0.4)%,平衡时高达(93.1±0.7)%,IC值为0.87 mg/mL,吸附容量高达(18.376±0.066)mg/g,吸附符合Freundlich等温线模型和准二级动力学模型;其对亚硝胺合成的阻断率达到(65.2±2.1)%。由此说明麒麟菜残渣超微粉具有突出的理化性质、亚硝酸钠吸附能力和亚硝胺合成阻断率,适用于开发亚硝酸钠吸附剂。

Sodium nitrite is one of the most toxic food additives,and insoluble dietary fiber(IDF)can scavenge sodium nitrite.To reduce the harm caused by sodium nitrite to humans,this paper studied the chemical composition of Eucheuma sp.waste residue after extracting carrageen.Then the Eucheuma sp.waste residue was pulverized to be ultrafine powder,and their hydration characteristics and cation exchange capacity were determined.On this basis,their capacity to adsorb sodium nitrite and ability to block nitrosamine synthesis under the simulated gastric juice in vitro was investigated.Results showed that the IDF content of Eucheuma sp.waste residue was up to 90.1%.Their maximum water holding capacity and expansion capacity reached(5.610×0.06)g/g and(5.670×0.30)mL/g,respectively,and their maximum cation exchange capacity was(0.320×0.02)mmol NaOH/g.More interestingly,they could adsorb(70.00x0.4)%sodium nitrite within 5 min and the adsorption ratio reached(93.10×0.7)%after equilibrium.The IC 50 value was 0.87 mg/mL,and the adsorption capacity was up to(18.3760×0.066)mg/g.The adsorption accorded with the Freundlich isotherm model and pseudo-second-order kinetic model.Furthermore,their blocking rate of nitrosamine synthesis reached(65.20×2.1)%.In conclusion,the ultrafine powder of Eucheuma sp.waste residue has excellent physicochemical properties,sodium nitrite adsorption capacity,and nitrosamine synthesis blocking ability,thereby it is suitable for developing sodium nitrite scavengers.