氢化物原子荧光光谱法测定黄芪中硒含量的方法优化

Optimization of the Methods for the Determination of Selenium Content in Astragalusmemeranaceus by Hydride Generation Fluorescence Spectrometry

探索氢化物原子荧光光谱法测定黄芪中总硒的仪器参数和载流条件,优化样品消解方式,以建立氢化物原子荧光光谱法测定黄芪中总硒的最佳的条件。以黄芪成分分析标准物质为材料,通过单一变量法,考察确定最优仪器条件、载流试剂条件和消解条件,并最终在最优条件下测定黄芪中硒含量。结果表明:通过单一变量法,最终确立氢化物原子荧光光谱法测定黄芪中总硒的最佳的条件为:负高压300V、灯电流80mA,试剂条件为载流盐酸浓度2%、硼氢化钾溶液浓度12g·L^(-1),消解条件为盐酸体积5mL、消解时间90min,在此条件下测得的黄芪中硒含量为0.135mg·kg^(-1)。该法简单、准确度高,适用于黄芪中硒含量的测定。

The instrument parameters and current-carrying conditions for the determination of total selenium in Astragalus memeranaceus by hydride generation fluorescence spectrometry were explored,and the sample digestion methods were optimized,in order to establish the best conditions for the determination of total selenium in Astragalus memeranaceus by hydride generation fluorescence spectrometry.By taking the standard reference material of component analysis of Astragalus memeranaceus as the material,the optimal instrument conditions,current-carrying reagent conditions and digestion conditions were investigated and determined by using the single variable method,and finally the content of selenium in Astragalus memeranaceus was determined under the optimal conditions.The results showed that the optimum conditions for the determination of total selenium in Astragalus memeranaceus by hydride generation fluorescence spectrometry were as follows:the negative high voltage was 300 V,the lamp current was 80 mA,the reagent conditions were that the concentration of current-carrying hydrochloric acid being 2%,and the concentration of potassium borohydride solution being 12 g·L^(-1),the digestion conditions were that the volume of hydrochloric acid being 5 mL,and the digestion time being 90 min.Under this condition,the content of selenium in Astragalus memeranaceus was 0.135 mg·kg^(-1).The method was simple,accurate and suitable for the determination of selenium content in Astragalus memeranaceus.