枇杷低温胁迫下的转录因子分析

Analysis of the Transcriptional Factors in Loquat under Low Temperature Stress

以一年生“冠玉”枇杷嫁接苗为研究对象,在4℃条件下处理叶片4 h,挖掘枇杷低温胁迫下的转录组数据,通过plantTFcat网站分析转录因子(Transcriptional factors,TFs)变化规律,并下载网站中所有12类果树,以拟南芥转录因子数据为对照,探讨枇杷低温反应的分子机制.结果表明:枇杷叶片在低温下有30个CAMTA3基因在内的53类1308个TFs胁迫表达,其中CBF基因表达最为快速;TFs种类及总数在枇杷和其他12类果树间差异大,不同物种存在缺失的TFs,bHLH、ERF、NAC类TFs及ICE-CBF-COR途径在枇杷低温胁迫中发挥重要作用.说明植物冷胁迫快慢两条途径均发挥了作用,但分子机理相对比较复杂.本研究结果为揭示枇杷低温响应的生理和分子调控机理提供理论依据,并为枇杷抗冻基因的挖掘和利用奠定基础.

In this study,the grafted seedlings of the"Guanyu"loquat for one year were treated at 4℃for 4 hours,and the transcriptome data was extracted from the loquat under the low temperature stress.The change law of transcriptional factors(TFs)was analyzed through the plantTFcat website,and all 12 kinds of fruit trees in the website were downloaded.Taking the Arabidopsis transcriptional factor data as a control,the molecular mechanism of the loquat low temperature response was discussed.The results showed that 1308 TFs of 53 categories including 30 CAMTA3 genes were expressed in loquat leaves under the low temperature stress,of which the CBF genes were expressed most rapidly.The types and the total number of TFs differed greatly between the loquat and the other 12 fruit trees,and TFs were missing in different species.The bHLH,ERF,NAC TFs and ICE-CBF-COR pathways all play an important role in the loquat under the low temperature stress,indicating that both fast and slow pathways of the plant cold stress play a role,but the molecular mechanism is relatively complex.The results of this study not only provide a theoretical basis for revealing the physiological and molecular regulation mechanism of the loquat low temperature response,but also lay a foundation for the extraction and utilization of loquat anti-freezing genes.