子宫内膜异位症不孕症患者血清miR-203a-3p、SOCS1、SOCS3表达

Expression of the serum miR-203a-3p,cytokine signaling 1,and suppressor of cytokine signaling 3 of infertility women with endometriosis

目的:探究子宫内膜异位症不孕患者血清中miR-203a-3p、细胞因子信号转导抑制因子1(SOCS1)、SOCS3表达及临床意义。方法:收集本院2015年1月-2019年1月行手术治疗的子宫内膜异位症不孕患者,经术后随访24个月均妊娠者76例(异位组),根据妊娠结局良好组(41例)及不良组(35例);本院体检健康女性76例为对照组。实时荧光定量PCR仪检测血清中miR-203a-3p表达,酶联免疫吸附试验测定血清SOCS1、SOCS3水平。Pearson法分析异位组miR-203a-3p与SOCS1、SOCS3的相关性。用受试者工作特征曲线(ROC)分析血清miR-203a-3p、SOCS1、SOCS3水平对子宫内膜异位症不孕的诊断价值。结果:与对照组相比,异位组血清miR-203a-3p水平较高,血清SOCS1、SOCS3水平较低;与Ⅰ/Ⅱ期相比,Ⅲ/Ⅳ期患者血清miR-203a-3p水平较高,SOCS1、SOCS3水平较低(均P<0.05)。Pearson分析显示,miR-203a-3p与SOCS1、SOCS3均呈负相关关系(P<0.05)。miR-203a-3p、SOCS1、SOCS3诊断子宫内膜异位症不孕症的曲线下面积(AUC)分别为0.866、0.818、0.806,三者联合诊断的AUC为0.929,特异性为90.8%,敏感度为84.2%。异位组中,与妊娠结局良好组相比,不良组患者血清miR-203a-3p水平较高,SOCS1、SOCS3水平较低(均P<0.05)。结论:子宫内膜异位症不孕患者血清miR-203a-3p水平异常升高,血清SOCS1、SOCS3水平异常降低,miR-203a-3p、SOCS1、SOCS3对子宫内膜异位症不孕症有一定诊断价值。

Objective:To explore the expression and clinical significance of the serum miR-203a-3p,suppressor of cytokine signaling 1(SOCS1),and suppressor of cytokine signaling 3(SOCS3)of infertility women with endometriosis.Methods:During January 2018 to October 2019,76 infertility women with endometriosis who had given surgical treatment and had been pregnancy in postoperative 24 months of follow up were included in study group,and were divided into group A(41 cases with normal pregnancy outcomes)and group B(35 cases with adverse pregnancy outcomes)according to the results of pregnancy.Another 76 healthy women who had accepted healthy physical examination were included in control group during the same period.The expression of miR-203a-3p in serum of the women in these groups was detected by a real-time fluorescent quantitative PCR instrument.The levels of the serum SOCS1 and SOCS3 of the women in these groups were determined by enzyme-linked immunosorbent assay(ELISA).The correlation between the serum miR-203a-3p level of the women in study group and their serum SOCS1 and SOCS3 levels was analyzed by Pearson method.The diagnostic value of the serum miR-203a-3p,SOCS1,SOCS3 levels for endometriosie and infertility of the women was analyzed by receiver operating characteristic(ROC)curve.Results:The serum level of miR-203a-3p of the women in the study group was significantly higher than that of the women in the control group,while the serum levels of SOCS1 and SOCS3 of the women in the study group were significantly lower.The serum level of miR-203a-3p of the women with endometriosis stageⅢ/Ⅳwas significantly higher than that of the women with endometriosis stageⅠ/Ⅱ,while the serum levels of SOCS1 and SOCS3 of the women with endometriosis stageⅢ/Ⅳwere significantly lower(all P<0.05).Pearson analysis showed that miR-203a-3p level of the women in the study group was negatively correlated with their SOCS1 and SOCS3 levels(P<0.05).The area under curve(AUC)of the miR-203a-3p level,SOCS1 level,and SOCS3 level of the women in the study group for diagnosing their endometriosis and infertility were 0.866,0.818,and 0.806,respectively.The AUC,the specificity,and the sensitivity of the combined of the levels of miR-203a-3p,SOCS1,and SOCS3 the women in the study group for diagnosing their endometriosis and infertility were 0.929,90.8%,and 84.2%,respectively.In the study group,the serum level of miR-203a-3p of the women in group B was significantly higher than that of the women in group A,while the levels of SOCS1 and SOCS3 of the women in group B were significantly lower(all P<0.05).Conclusion:The level of the serum miR-203a-3p of the infertility women with endometriosis increases abnormally,and the levels of their serum SOCS1 and SOCS3 decrease abnormally.The levels of MiR-203a-3p,SOCS1,and SOCS3 of the women have certain diagnostic value for their endometriosis and infertility.