血清外泌体、胎盘来源外泌体和外泌体miR-210与子痫前期的相关性

Correlation of serum exosomes,placental exosomes and exosomal miR-210 with preeclampsia

目的 探讨血清外泌体、胎盘来源外泌体[胎盘碱性磷酸酶(PLAP)]和外泌体微小RNA-210(miR-210)在子痫前期(PE)患者中的变化及临床意义。方法 选取PE患者30例(PE组)和健康孕妇30例(正常妊娠组),收集所有对象孕早期、孕中期和孕晚期的血清样本,检测血清外泌体数量、PLAP水平和外泌体miR-210相对表达量。采用Pearson相关分析评估PLAP与miR-210的相关性。结果 正常妊娠组和PE组血清外泌体数量、PLAP水平和外泌体miR-210相对表达量均随孕期逐步升高。PE组不同孕期血清外泌体数量、PLAP水平和外泌体miR-210相对表达量均高于正常妊娠组(P<0.05)。PLAP水平与外泌体miR-210相对表达量呈正相关(r=0.668,P<0.01)。结论 PE患者血清外泌体数量、PLAP水平和外泌体miR-210相对表达量在孕早期已发生变化,这3项指标或可作为预测和早期诊断PE的生物标志物。

Objective To investigate the changes and roles of serum exosomes,placental exosomes [placental alkaline phosphatase(PLAP)] and exosomal microRNA-210(miR-210) in pregnant women during preeclampsia(PE) pregnancy. Methods Totally,30 patients with PE and 30 healthy pregnant women were enrolled. All serum samples were collected in the first trimester,the second trimester and the third trimester of pregnancy. The amount of serum exosomes,the level of PLAP and the expression of exosomal miR-210 were determined. The correlation between PLAP and miR-210 was analyzed using Pearson analysis. Results The amount of serum exosomes,the level of PLAP and miR-210 were increased with the progress of pregnancy. Compared with healthy pregnant women,the amount of serum exosomes,the level of PLAP and miR-210 in PE group were higher in different trimesters of pregnancy(P<0.05). The correlation between the level of PLAP and exosomal miR-210 was positive(r=0.668,P<0.01). Conclusions The amount of serum exosomes,the level of PLAP and exosomal miR-210 in PE group change from the first trimester of pregnancy,and they play roles in the early prediction and diagnosis of PE.