异烟肼⊂β-环糊精包合物的制备及包合研究实验

Experiment of the preparation of inclusion compound of isoniazide⊂β-cyclodextrin and study on their inclusion interaction

综合性实验就是在实验过程中,采用多步反应、多种实验手段和多种实验技术来协同完成实验目的的实验。包括异烟肼的制备,异烟肼和β-环糊精包合物的制备,异烟肼和β-环糊精包合作用的研究三部分。通过异烟肼的制备、异烟肼与β-环糊精包合物的制备和包结常数的研究,让学生了解质谱、核磁、熔点仪、紫外可见光谱仪、荧光光谱仪的使用和数据处理,使学生能够掌握经典药物异烟肼的合成,异烟肼与β-环糊精包合物的制备及其包结常数的研究方法,涵盖了有机合成、仪器分析及物理化学等不同学科的知识,可以作为一个综合性实验对高年级化学类专业和制药专业的本科生及研究生开放。

Comprehensive experiments are involved reactions of several steps,various experimental techniques to accomplish the final experimental purpose.This experiment includes the synthesis of isoniazid,the preparation of inclusion compound of isoniazid andβ-cyclodextrin and the research on their inclusive interaction.Students can master the synthesis of isoniazid,understand the usage and data processing of MS,NMR,melting point instrument,UV-Vis and fluorescence spectrometer,further to learn the preparation and research method of inclusion compounds.This experiment covers the knowledge of organic synthesis,instrument analysis and physical chemistry,can be used as a comprehensive experiment for senior undergraduates or graduate student for the major of chemistry or pharmacy.