牛磺酸对人宫颈癌细胞侵袭转移的抑制作用及机制研究

Inhibitory Effect of Taurine on Invasion and Metastasis of Human Cervical Cancer Cells and Its Mechanism

目的探讨牛磺酸(Tau)对人宫颈癌细胞侵袭转移的影响及分子机制。方法采用20~160 mmol/L不同浓度Tau处理宫颈癌Hela细胞24~72 h,CCK8检测细胞增殖活性,Transwell小室检测细胞侵袭与迁移能力,Western blot检测蛋白表达。结果终浓度为40~160 mmol/L的Tau对人宫颈癌细胞增殖和侵袭转移有不同程度的抑制作用(P<0.05),且呈时效和量效关系;同时Tau能增加上皮-间质转化(EMT)代表上皮特征的E-cadherin表达,下调代表间质特征的N-cadherin,Vimentin蛋白及基质金属蛋白酶(MMPs)表达;抑制AKT/GSK-3β通路及降低β-catenin蛋白含量。结论Tau具有抑制人宫颈癌细胞侵袭转移与EMT的作用,其机制可能与AKT/GSK-3β/β-catenin信号通路有关。

Objective To investigate the effect and mechanism of taurine(Tau)on invasion and metastasis of human cervical cancer cells.Methods Cervical cancer Hela cells were treated with Tau at different concentrations for 24~48 h,the proliferative activity of the cells was detected by CCK8;the ability of cell migration and invasion were detected by Transwell assay;Western blot was used to detect protein expression in the cells.Results The results showed that Tau(final concentration 40~160 mmol/L)can inhibit the proliferation,invasion and migration of cervical cancer cells to varying degrees(P<0.05),And showed a certain dose-effect relationship;Meanwhile,Tau increased the expression of E-cadherin,a marker of epithelial-mesenchymal transformation(EMT),and down-regulated the expression of N-cadherin,Vimentin and matrix metalloproteinases(MMPs),inhibited the AKT/GSK-3βpathway and reduced theβ-catenin protein.Conclusion Tau can inhibit EMT,invasion and metastasis of cervical cancer cells,and the mechanism might be related to AKT/GSK-3β/β-catenin signaling pathway.