E2F转录因子在子宫内膜癌中表达及意义的生物信息学分析

Bioinformatics analysis of the expression and significance of E2F transcription factor in endometrial cancer

目的通过生物信息学方法,分析E2F转录因子在子宫内膜癌中的表达及意义。结合表达差异基因和富集通路,预测子宫内膜癌的潜在生物标志物。方法使用蛋白质-蛋白质相互作用(PPI)、共表达网络,分析E2F在子宫内膜癌中的表达。筛选癌组织和癌旁组织差异表达的E2F基因,对差异基因进行生物过程(GO)分析和KEGG富集分析。单因素Cox分析筛选预后相关的E2F基因。结果本研究筛选了6个差异基因及2个预后基因,差异基因主要参与细胞周期调节、癌症相关信号通路。有助于理解E2F在子宫内膜癌中的发病机制及作用,为寻找子宫内膜癌的治疗靶点提供思路。结论E2F与子宫内膜癌发生、发展密切相关。E2F1/E2F2/E2F3/E2F5/E2F7/E2F8是子宫内膜癌诊断的候选分子标志物。E2F1/E2F3是子宫内膜癌潜在的预后生物标志物。

Objective To analyze the expression and significance of E2F transcription factor in endometrial cancer by bioinformatic methods.To predict potential biomarkers for endometrial cancer by combining differentially expressed genes and enriched pathways.Methods The expression of E2F in endometrial cancer was analyzed using proteinprotein interaction(PPI)and co-expression networks.The differentially expressed E2F genes in cancer tissues and adjacent tissues were screened,and biological process(GO)analysis and KEGG enrichment analysis were performed on the differential genes.Results The study screened six differential genes and two prognostic genes.The differential genes were mainly involved in cell cycle regulation and cancer-related signaling pathways.It contributed to a better understanding of the pathogenesis and role of E2F in endometrial cancer,as well as treatment targets for endometrial cancer.Conclusion Endometrial carcinogenesis and development are tightly linked to E2F.E2F1/E2F2/E2F3/E2F5/E2F7/E2F8 may be candidate biomarkers for diagnosis of endometrial cancer and E2F1/E2F3 are potential prognosis biomarkers of endometrial cancer.