呼吸道病原体五联检在上呼吸道感染患儿中的应用

Application of five-joint inspection of respiratory pathogens in children with upper respiratory tract infection

目的探讨呼吸道病原体五联检在上呼吸道感染(URTI)患儿中的应用价值。方法选择2021年2月至5月于陆丰市第三人民医院治疗的80例URTI患儿,采集患儿静脉血2 ml,2 h内分离血清,采用胶体金法测定血清中肺炎支原体(MP)IgM抗体(MP-IgM)、肺炎衣原体(CP)IgM抗体(CP-IgM)、呼吸道合胞病毒(RSV)IgM抗体(RSV-IgM)、腺病毒(ADV)IgM抗体(ADV-IgM)、柯萨奇病毒B组(CoxB)IgM抗体(CoxBIgM)。统计80例URTI病毒检测阳性率,计算MP-IgM、CP-IgM、RSV-IgM、ADV-IgM、CoxB-IgM单项及联合检出阳性率。结果80例URTI病毒检测阳性率为57.50%(46/80)。单项病原体检出阳性率为48.75%(39/80),其中MP-IgM为18.75%(15/80)、CP-IgM为12.50%(10/80)、RSV-IgM为8.75%(7/80)、ADV-IgM为5.00%(4/80)、CoxB-IgM为3.75%(3/80)。混合感染检出阳性率为8.75%(7/80),其中二项病原体检出阳性率为5.00%(4/80),MP-IgM+CP-IgM为2.50%(2/80)、RSV-IgM+CoxB-IgM为1.25%(1/80)、MP-IgM+ADVIgM为1.25%(1/80);三项病原体检出阳性率为2.50%(2/80),其中MP-IgM+CP-IgM+ADV-IgM为1.25%(1/80)、CP-IgM+RSV-IgM+CoxB-IgM为1.25%(1/80);四项病原体(MP-IgM+CP-IgM+RSV-IgM+ADV-IgM)检出阳性率为1.25%(1/80)。结论针对URTI患儿,应尽早进行呼吸道病原体五联检检测,检测病原体IgM,以正确诊断并指导临床及时治疗,避免抗菌药物的滥用。

Objective To investigate the application value of five-joint inspection of respiratory pathogens in children with upper respiratory tract infection(URTI).Methods A total of 80 URTI children treated in Lufeng Third People’s Hospital from February to May 2021 were selected,2 ml of venous blood was collected from the children,and the serum was separated within 2 hours.The colloidal gold method was used to determine the serum levels of Mycoplasma pneumoniae(MP)IgM antibody(MP-IgM),Chlamydia pneumoniae(CP)IgM antibody(CP-IgM),and respiratory syncytial virus(RSV)IgM antibody(RSV-IgM),adenovirus(ADV)IgM antibody(ADV-IgM),Coxsackie virus group B(CoxB)IgM antibody(CoxB-IgM).The positive rate of URTI virus detection in 80 cases was counted,and the positive rate of MP-IgM,CP-IgM,RSV-IgM,ADV-IgM,CoxB-IgM and combined detection was calculated.Results The positive rate of URTI virus in 80 cases was 57.50%(46/80).The positive rate of single pathogen was 48.75%(39/80),in which MP-IgM was 18.75%(15/80),CP-IgM was 12.50%(10/80),RSV-IgM was 8.75%(7/80),ADV-IgM was 5.00%(4/80)and CoxB-IgM was 3.75%(3/80).The positive rate of mixed infection was 8.75%(7/80),of which the positive rate of two pathogens was 5.00%(4/80),of which MP-IgM+CP-IgM was 2.50%(2/80),RSVIgM+CoxB-IgM was 1.25%(1/80),MP-IgM+ADV-IgM was 1.25%(1/80).The positive rate of three pathogens was 2.50%(2/80),of which MP-IgM+CP-IgM+ADV-IgM was 1.25%(1/80),and CP-IgM+RSV-IgM+CoxB-IgM was 1.25%(1/80).The positive rate of four pathogens(MP-IgM+CP-IgM+RSV-IgM+ADV-IgM)was 1.25%(1/80).Conclusion For URTI children,the five-joint inspection of respiratory pathogens should be carried out as soon as possible to detect pathogen IgM,so as to correctly diagnose and guide clinical ti mely treatment and avoid the abuse of antibiotics.