胃复春对Hp感染萎缩性胃炎大鼠模型胃黏膜组织IL-6/JAK2/STAT3信号通路的影响

Effect of Weifuchun on IL-6/JAK2/STAT3 signaling pathway in gastric mucosal tissues of model rats with Hp infection and atrophic gastritis

目的分析胃复春对幽门螺杆菌(Hp)感染慢性萎缩性胃炎(CAG)大鼠模型胃黏膜组织白细胞介素-6(IL-6)/Janus蛋白酪氨酸激酶2(JAK2)/信号转导子与激活子3(STAT3)信号通路的影响。方法选取雄性健康大鼠45只,随机分为空白对照组12只及造模组33只;采用Hp悬液灌胃及水杨酸钠、乙醇混合液灌胃造模,造模14周末,观察Hp感染CAG模型成功与否;造模成功后,将造模组随机分为模型对照组、高剂量组、低剂量组,模型对照组给予10 ml/kg生理盐水灌胃,高剂量组给予1.56 g/kg胃复春灌胃,低剂量组给予0.78 g/kg胃复春灌胃,空白对照组仅正常饲养,给药4周;比较4组胃黏膜组织Hp培养阳性率及病理评分、IL-6、JAK2、STAT3、核因子-κB(NF-κB)信使核糖核酸(mRNA)相对表达水平。结果造模的3组胃黏膜组织Hp培养阳性率均高于空白对照组(P<0.05)。造模的3组胃窦及胃体黏膜组织病理评分、胃黏膜组织IL-6、JAK2、STAT3、NF-κB mRNA相对表达水平均高于空白对照组(P<0.05),且模型对照组高于高剂量组及低剂量组(P<0.05),低剂量组则高于高剂量组(P<0.05)。结论胃复春对Hp感染CAG大鼠疗效显著,其作用机制可能与抑制IL-6/JAK2/STAT3信号通路及NF-κB的表达有关。

OBJECTIVE To observe the effect of Weifuchun on interleukin-6(IL-6)/Janus protein tyrosine kinase 2(JAK2)/signal transducer and activator of transcription 3(STAT3)signaling pathway in gastric mucosal tissues of model rats with Helicobacter pylori(Hp)infection complicated with chronic atrophic gastritis(CAG).METHODS Totally 45 healthy male rats were selected and randomly divided into the blank control group with 12 rats and the modeling group with 33 rats.The model was established by gavage of Hp suspension and mixture of sodium salicylate and ethanol.The model of Hp infection with CAG was observed at the end of the 14 th week of modeling,the rats of the model group were randomly divided into the control group,the high-dose group and the low-dose group after the successful modeling.The model control group was given 10 mL/kg normal saline by gavage,and the high-dose group was given 1.56 g/kg Weifuchun by gavage,and the low-dose group was given 0.78 g/kg Weifuchun by gavage,and the blank control group was only fed normally,and all rats were given 4 weeks of administration.The positive rate of Hp culture,pathological scores,messenger ribonucleic acid(mRNA)relative expression levels of IL-6,JAK2,STAT3 and nuclear factor-κB(NF-κB)in gastric mucosal tissues were compared among the four groups.RESULTS The positive rate of Hp in gastric mucosal tissues of the three modeling groups was significantly higher than that of the blank control group(P<0.05).The pathological scores of gastric antrum and gastric body mucosal tissues and mRNA relative expression levels of gastric mucosal tissue IL-6,JAK2,STAT3 and NF-κB were significantly higher in the three modeling groups than in the blank control group(P<0.05),which were significantly higher in the model control group than in the high-dose group and the low-dose group,significantly higher in the low-dose group than in the high-dose group(P<0.05).CONCLUSION Weifuchun can achieve remarkable therapeutic effect on the rats with Hp infection and CAG,and the action mechanisms may be related to its inhibition of expression of IL-6/JAK2/STAT3 signaling pathway and NF-κB.