HPV感染对口咽癌患者癌组织免疫微环境及T细胞效应分子表达水平和存活时间的影响

Influence of HPV infection on immune microenvironment and expression levels of T cell effector molecules in cancer tissues and survival time of patients with oropharyngeal cancer

目的 分析人乳头瘤病毒(HPV)感染对口咽癌患者癌组织免疫微环境、T细胞效应分子表达水平和存活时间的影响。方法 选择2007年1月-2017年1月淄博市第一医院收治的98例口咽癌患者为研究对象,检测患者HPV感染情况,比较HPV阳性和HPV阴性口咽癌患者癌组织免疫微环境、T细胞效应分子表达水平,并分析影响口咽癌患者生存预后的危险因素。结果 98例口咽癌患者肿瘤标本检测结果显示,HPV阳性26例,感染率为26.53%,HPV阳性组癌组织CD_(8)^(+) T细胞亚群占比高于HPV阴性组(P<0.05),HPV阳性组患者T细胞效应分子干扰素-γ(IFN-γ)、颗粒酶B(Granzyme B)、白细胞介素-2(IL-2)相对表达量高于HPV阴性组患者(P<0.05),多因素Cox风险回归模型分析结果,HPV感染是影响口咽癌患者生存预后的因素,HPV阴性患者死亡风险增加17.110倍。结论 HPV感染是影响口咽癌患者生存预后的重要因素,HPV阴性患者死亡风险较HPV阳性患者显著提高,其机制可能与HPV阳性患者CD_(8)^(+) T细胞及效应分子IFN-γ、Granzyme B表达水平上升有关,提高免疫细胞浸润可能有利于口咽癌患者治疗效果。

OBJECTIVE To observe the influence of human papillomavirus(HPV)infection on the immune microenvironment and expression levels of T cell effector molecules in cancer tissues and survival time of the patients with oropharyngeal cancer.METHODS Totally 98 patients with oropharyngeal cancer who were treated in the Zibo First Hospital from Jan 2007 to Jan 2017 were recruited as the study objects,the prevalence of HPV infection was detected,the immune microenvironment and expression levels of T cell effector molecules in cancer tissues were observed and compared between the HPV-positive and HPV-negative oropharyngeal cancer patients,and the risk factors for survival prognosis of the oropharyngeal cancer patients were analyzed.RESULTS The result of tumor specimens test for the 98 oropharyngeal cancer patients showed that 26 were positive for HPV,with the infection rate 26.53%.The percentages of CD_(8)^(+)T cell subsets in cancer tissues were higher in the HPV-positive group than in the HPV-negative group(P<0.05).The relative expression levels of T cell effector molecules interferon-γ(IFN-γ),granzyme B(granzyme B)and interleukin-2(IL-2)of the HPV-positive group were higher than those of the HPV-negative group(P<0.05).The result of multivariate Cox risk regression model analysis showed that the HPV infection was an influencing factor for the survival prognosis of the oropharyngeal cancer patients,the death risk of the HPV-negative patients was increased by 17.110 times.CONCLUSION The HPV infection is a leading factor affecting the survival prognosis of the oropharyngeal cancer patients,the risk of death of the HPV-negative patients is remarkably higher than that of the HPV-positive patients,the mechanisms may be associated with the rise of expression levels of CD_(8)^(+)T cell and effector molecules IFN-γ,Granzyme B in the HPV-positive patients,and the enhancement of immune cell infiltration may facilitate the curative effect of the oropharyngeal cancer patients.