鸡内金对大鼠肾草酸钙结石的防治作用研究

Preventive effects of Galli gigerii endothelium corneum on renal calculus in rats

目的 通过研究鸡内金对大鼠肾草酸钙结石模型的作用,探讨鸡内金对大鼠肾草酸钙结石的防治作用。方法 选用SPF级雄性大鼠40只,按体重排序后采用随机数字表法随机分为正常组、模型组和鸡内金高、中、低剂量组,每组8只。正常组给予正常饮水和饲料,模型组给予造模剂(1%乙二醇饮水+上午0.2 mL/只2%氯化铵灌胃),鸡内金高剂量组(5.00 g/kg体重)、中剂量组(2.50 g/kg体重)、低剂量组(1.25 g/kg体重)同时在下午分别按0.2 mL/100 g体重灌胃,连续4周。试验结束后测定各组大鼠24 h排尿量和尿钙(Ca)、尿磷(P)的含量,以及肾组织过氧化氢酶(CAT)、丙二醛(MDA)的含量,分析各组大鼠血清CAT、MDA、Ca、P、肌酐(Cr)、BUN含量,观察肾组织病理学变化。结果 与模型组比较,鸡内金高剂量组肾CAT[(6.1±1.3) U/mg vs.(4.1±1.2) U/mg,P<0.05]、血清CAT[(29.8±1.5) U/mL vs.(26.6±1.3) U/mL,P<0.01]升高,鸡内金高、中、低剂量组肾MDA[(15.71±0.21) nmol/mg、(16.84±0.18) nmol/mg、(17.29±0.11) nmol/mg vs.(17.59±0.19) nmol/mg,均P<0.01]含量均降低,鸡内金高剂量组血清Cr[(46.5±3.3) mmol/mL vs.(52.1±2.7) mmol/mL,P<0.01]降低。与模型组比较,鸡内金各剂量组肾组织病理损伤有所减轻。结论 鸡内金能有效预防大鼠肾草酸钙结石,并保护肾功能。

Objective To study the effect of Galli gigerii endothelium corneum on kidney calcium oxalate stone in rat model,and to explore the preventive and therapeutic effect of Galli gigerii endothelium corneum on kidney calcium oxalate stone in rats.Methods Forty SPF male rats were randomly divided into normal group,model group,high-dose,medium-dose and low-dose groups.The normal group rats were given normal drinking water and feed,and the model group rats were given modeling agent(1%glycol water+0.2 mL/only 2%ammonium chloride to fill the stomach in the morning).The rats in high-dose(5.00 g/kg BW),medium-dose(2.50 g/kg BW),and low-dose(1.25 g/kg BW)GGEC groups fill the stomach with 0.2 mL/100 g BW at the same time in the afternoon for four weeks.The 24 h urine output,urinary calcium(Ca),urinary phosphorus content(P),and renal catalase(CAT),malondialdehyde content(MDA),serum CAT,MDA,Ca,P,creatinine(Cr),BUN contents were analyzed,and renal histopathological changes were observed at the end of the experiment.Results Compared with the model group,renal CAT[(6.1±1.3)U/mg vs.(4.1±1.2)U/mg,P<0.05]and serum CAT[(29.8±1.5)U/mL vs.(26.6±1.3)U/mL,P<0.01]significantly increased in the high-dose group,whereas renal MDA significantly decreased in the high-,medium-,and low-dose groups[(15.71±0.21)nmol/mg,(16.84±0.18)nmol/mg,(17.29±0.11)nmol/mg vs.(17.59±0.19)nmol/mg,all P<0.01],respectively.Serum Cr[(46.5±3.3)mmol/mL vs.(52.1±2.7)mmol/mL,P<0.01]significantly decreased in the high-dose group.Compared with the model group,the pathological damage of renal tissue in each dose group of GGEC was reduced.Conclusion The GGEC can effectively prevent calcium oxalate calculi and protect renal function in rats.