摘要
研究了锻造态Mg-Zn-Zr和Mg-Zn-Zr-Y镁合金在模拟体液中的pH值变化规律;按照ISO 10993的标准要求,分别制备了锻造态Mg-Zn-Zr/-Y合金不同浓度(100%、50%、10%)脐静脉血管内皮细胞(HUVEC)浸提液,通过体外溶血率测试了锻造态Mg-Zn-Zr/-Y镁合金的溶血率;通过CCK-8法体外细胞毒性试验及细胞形貌考察评价了锻造态Mg-Zn-Zr/-Y镁合金对HUVEC的细胞毒性。结果表明:锻造态Mg-Zn-Zr/-Y在SBF模拟体液中的腐蚀速度均高于纯镁的腐蚀速度;锻造态Mg-Zn-Zr镁合金溶血率为0.6%,锻造态Mg-Zn-Zr-Y镁合金溶血率为0.5%,不会导致严重溶血;锻造态Mg-Zn-Zr/-Y镁合金材料的浸提液浓度为10%时,对细胞的毒性较小,几乎不影响细胞的增殖。
In this paper,the pH values of forged Mg-Zn-Zr/-Y magnesium alloys simulated body fluids(SBF)are studied.The forged Mg-Zn-Zr/-Y alloy is prepared with different concentrations(100%,50%,10%)human umbilical vein endothelial cell(HUVEC)extract according to the ISO 10993 standard,and the hemolysis rate of the forged magnesium alloy is measured by in vitro hemolysis rate.The forging state Mg-Zn-Zr/-Y isevaluated by CCK-8 in vitro cytotoxicity test and morphology observation cytotoxicity in HUVEC.The results show that the corrosion rate of forged Mg-Zn-Zr/-Y in SBF simulated body fluids is higher than that of pure magnesium.The hemolysis rate of Mg-Zn-Zr forged magnesium alloy is 0.6%,and which of forged Mg-Zn-Zr-Y magnesium alloy is 0.5%,indicating that forged magnesium alloy will not cause severe hemolysis.Forged Mg-Zn-Zr/-Y magnesium alloy materials with a concentration of 10%,the toxicity to cells is small.
作者
许东光
马春华
冷玉敏
杨浩
XU Dongguang;MA Chunhua;LENG Yumin;YANG Hao(College of Mechanical and Electronic Engineering,Nanyang Normal University,Nanyang 473061,China;College of Materials Science and Engineering,Chongqing University,Chongqing 400044,China;Henan Rare-earth Alloy Material Engineering and Engineering Research Center,Nanyang 473061,China;Chemistry and Pharmaceutical Engineering,Nanyang Normal University,Nanyang 473061,China)
出处
《生物化工》
2022年第4期29-33,43,共6页
Biological Chemical Engineering
基金
河南省科学技术厅科技攻关项目(212102210437)。
