高选择性、高灵敏的咔唑衍生物F^(-)荧光增强型探针

The Highly Selective and Sensitive Carbazole Derivative F^(-) Fluorescence-enhanced Probe

以4-羟基咔唑为原料,合成了一种含硅氧基团的咔唑衍生物(HCTC),通过核磁共振氢谱和高分辨质谱对其结构进行了表征,并对探针的F^(-)检测性能进行了研究。结果表明:HCTC能够利用F^(-)对硅氧键的高亲核性的特异性进攻,表现出对F^(-)的化学反应型荧光探针特性;当加入F^(-)以后,该探针溶液在波长433 nm处出现新的荧光发射峰,并伴随轻微的红移现象,而加入其他常见的阴离子都不能引起类似的现象,HCTC对F^(-)的检测限相对较低(0.21μmol/L);将HCTC探针制备成荧光试纸,它能够对含少量乙腈的水溶液中的F^(-)具有荧光检测性能。HCTC是一种高选择性、高灵敏度的F^(-)荧光增强型探针。

A carbazole derivative (HCTC) containing siloxy groups was synthesized using 4-hydroxycarbazole as the raw material. The structure was characterized with 1 H NMR and MS, and the F^(-) detection performance of the probe was studied. The results showed that HCTC could take advantage of the nucleophilicity of F^(-) to specifically attack the silicon-oxygen bond, showing the characteristics of a chemically reactive fluorescent probe towards F^(-) . Fluorescence test results showed that when F^(-) was added, a new fluorescence emission peak appeared in the probe solution at the wavelength of 433 nm, accompanied by a slight red shift, while the addition of other common anions could not cause fluorescence enhancement. In addition, the detection limit of F^(-) by HCTC was relatively low (0.21 μmol/L). Finally, the HCTC probe was prepared into a fluorescent test paper, which could detect F^(-) in an aqueous solution containing a small amount of acetonitrile. HCTC can be a fluorescence-enhanced probe with high selectivity and sensitivity.