PTD-FNK蛋白对雄性小鼠生理功能的影响

Effects of PTD-FNK Protein on Physiological Function of Male Mice

[目的]探究PTD-FNK蛋白对雄性小鼠生理功能的影响。[方法]将16只8周龄健康雄性C57BL/6J小鼠随机分为对照组(n=8)和PTD-FNK蛋白组(n=8),对照组腹腔注射生理盐水,PTD-FNK蛋白组腹腔注射300μg/(kg·BW)PTD-FNK蛋白,每天腹腔注射给药1次,连续给药7 d;给药期间每天记录小鼠的体重、体温、血糖、采食量、饮水量,计算试验期间总采食量、总饮水量、体增重;于试验第1天和最后1天测量鼻肛距,计算试验前后Lee′s指数;试验结束立即处死小鼠,称量心脏、肾脏、肝脏、脾脏和睾丸重量,计算脏器指数;分离小鼠附睾,制备精子悬液,测定精子活力和质膜完整率;利用qPCR法检测睾丸组织中细胞凋亡相关基因Bax和Caspase-3、氧化应激相关基因SOD和GPX1、内分泌相关基因STAR、17β-HSD的mRNA相对表达量。[结果]与对照组相比,PTD-FNK蛋白组小鼠总采食量极显著(P<0.001)下降,总饮水量显著(P<0.05)下降;Lee′s指数、体温、体重、血糖无显著(P>0.05)变化。肝脏指数显著(P<0.05)下降,其他脏器指数无显著(P>0.05)变化。精子活力极显著(P<0.01)升高,精子质膜完整率无显著(P>0.05)变化。睾丸组织中Bax基因mRNA相对表达量极显著(P<0.01)升高,Caspase-3基因mRNA相对表达量无显著(P>0.05)变化,SOD基因mRNA相对表达量极显著(P<0.01)升高,GPX1基因mRNA相对表达量无显著(P>0.05)变化,17β-HSD、STAR基因的mRNA相对表达量无显著(P>0.05)变化。[结论]PTD-FNK蛋白可以抑制雄性小鼠的采食、饮水及肝脏的生长发育,提高小鼠的精子质量。

[Objective]The purpose of this study was to investigate the effects of PTD-FNK protein on physiological function of male mice.[Method]A total of 168-week-old healthy male C57BL/6J mice were randomly divided into control group(n=8)and PTD-FNK protein group(n=8).The PTD-FNK protein group was intraperitoneally injected with 300μg/(kg·BW)PTD-FNK protein once a day for consecutive 7 days,and the control group was intraperitoneally injected with normal saline.During the experimental period,the body weight,body temperature,blood glucose,feed intake and water intake of the mice were recorded every day,and the total feed intake,total water intake and weight gain were calculated.The body length(from nose to anus)of the mice was measured at the first and last day of the experiment,respectively,and the Lee′s index before and after the experiment was calculated,respectively.At the end of the experiment,the mice were sacrificed immediately,the heart,kidney,liver,spleen and testis were weighed,and the corresponding organ indexes were calculated.The epididymis of the mice was isolated,sperm suspension was prepared,and the sperm viability and plasma membrane integrity were determined.The mRNA relative expression levels of cell apoptosis related genes Bax and Caspase-3,oxidative stress related genes SOD and GPX1,and endocrine related genes STAR and 17β-HSD were assessed by using qPCR.[Result]The total feed intake and total water intake of the PTD-FNK protein group were extremely significantly(P<0.001)and significantly(P<0.05)decreased compared with those of the control group,respectively,while no significant(P>0.05)differences in Lee′s index,body temperature,body weight and blood glucose were observed between the control group and the PTD-FNK protein group.The liver index of the PTD-FNK protein group was significantly(P<0.05)decreased in comparison to that of the control group,while no significant(P>0.05)differences in the other organ indexes were found.The PTD-FNK protein group had extremely significantly(P<0.01)higher sperm viability than the control group,while there was no significant(P>0.05)difference in sperm plasma membrane integrity between the control group and the PTD-FNK protein group.Extremely significantly(P<0.01)higher mRNA relative expression levels of Bax and SOD genes in testicle tissues were observed in the PTD-FNK protein group,while no significant(P>0.05)differences in the mRNA relative expression levels of Caspase-3,GPX1,17β-HSD and STAR genes were found between the control group and the PTD-FNK protein group.[Conclusion]PTD-FNK protein inhibited the feed intake,water intake as well as the growth and development liver of male mice,and improved the sperm quality.